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Triton X-100

laboratory grade

t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
Linear Formula:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS Number:
MDL number:
PubChem Substance ID:

Quality Level


laboratory grade





mol wt

micellar avg mol wt 80,000
average mol wt 625

aggregation number



0.2-0.9 mM (20-25°C)

transition temp

cloud point 65 °C
pour point ~7 °C


water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow



SMILES string




InChI key


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General description

Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.
Triton X-100 has hemolytic property and is used for DNA extraction.


Triton-X has been used:
  • In the permeabilization of cells for immunofluorescence staining
  • As a component of lysis buffer in western blot analysis
  • As a component of Tris-buffered saline for the preparation of cell sections in Immunogold labelling for electron microscope


5 mL in glass bottle
1 gal in poly bottle
5 gal in poly drum
1 L in poly bottle
100, 500 mL in poly bottle

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Legal Information

Tri-Chek is a trademark of Micro Essential Laboratory, Inc.
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Signal Word


Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids



Flash Point(F)

483.8 °F

Flash Point(C)

251 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

  1. What is the molecular weight of X100, Triton® X-100?

    Based on an average of 9-10 ethylene oxide units per molecule, the molecular weight of Triton X-100 is 625 g/mol.

  2. What is the concentration of X100, Triton® X-100?

    This product is 100% active ingredient.

  3. What concentration of X100,Triton® X-100, is typically used to lyse cells?

    Typically, a 0.1 % solution of Triton X-100 is sufficient for lysing cells.

  4. Does X100, Triton® X-100, have any UV absorbance?

    X100 does have UV absorbance at 280 nm, which will interfere with the UV determination of proteins.

  5. Can I autoclave X100, Triton® X-100?

    Aqueous solutions of Triton X-100 are stable to autoclaving. At certain concentrations the solutions may be cloudy but dispersible above the cloud point. However, the solution should clear with stirring upon cooling.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Growth and hydration of Triton X-100 micelles in monovalent alkali salts: a light scattering study
Molina-Bolivar JA, et al.
The Journal of Physical Chemistry B, 106(4), 870-877 (2002)


Enzymatic Assay of Cholesterol Oxidase

This procedure applies to products that have a specification for the enzymatic activity of cholesterol oxidase. This assay is NOT to be used to assay Cholesterol Oxidase from Schizophyllum commune (Discontinued Product Number C7274) and from Brevibacterium sp. (Discontinued Product Number C8153).

Solubilization of Membrane Proteins

This page shows how to solubilize membrane proteins with products from Cytiva.

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