T1503

Sigma-Aldrich

Trizma® base

Primary Standard and Buffer, ≥99.9% (titration), crystalline

Synonym(s):
Tris base, Trometamol, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris(hydroxymethyl)aminomethane
Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
Beilstein/REAXYS Number:
741883
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

description

aminopeptidase substrate

assay

≥99.9% (titration)

form

crystalline

pH

10.5-12

useful pH range

7 - 9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubility

water: soluble (678 g/l at 20 °C)

absorption

≤0.05 at 290 nm at 40%

Featured Industry

Diagnostic Assay Manufacturing

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.
40% (w/w) solution in water is clear and colorless.

Application

Trizma® base has been used:
  • as a component of H buffer (cell dissociation buffer)
  • for washing and saturation of wells in double sandwich ELISA immunoenzymatic technique
  • as an assay buffer for reconstitution of extracted and dried protein samples
  • to prepare Tris-HCl buffer that is used to stabilize proteins
  • as a buffer to extract carotenoid from tubers
  • as a component of sample buffer during protein extraction prior to western blotting
  • as a component of sample buffer for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
  • to prepare simulated body fluid (SBF) for calcium phosphate (CaP) resorption assay
  • as a buffer for polydopamine (PDA) deposition on stainless steel (SS) substrate

Packaging

5, 10, 25, 50 kg in poly drum
25, 100, 250, 500 g in poly bottle
1 kg in poly bottle

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Easily compare specifications for Trizma products with the Trizma specification table.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point F

Not applicable

Flash Point C

Not applicable

  1. What is the pH of Trizma® base, T1503?

    The pH of a 0.1 M aqueous solution of Trizma base will have a pH of approximately 10.4.

  2. What type of electrode should I use to measure the pH of Trizma® base T1503?

    We recommend the use of glass-calomel electrodes for use with Trizma base solutions through a pH range of 0-12 and a temperature range of -5 °C to 80 °C. Silver/silver chloride reference electrodes are not recommended for use with Trizma solutions that contain proteins.

  3. How can I use T1503 to make a Trizma® buffered solution?

    Trizma base may be used directly in preparing a buffer by adjusting the pH with a 0.1 to 1.0 M HCl or blended with Trizma hydrochloride.

  4. Can Product T1503, Trizma® base be used as a standard?

    Trizma base meets the requirements for a primary basimetric standard.

  5. Can I autoclave solutions that contain Product T1503, Trizma® base?

    Yes, Trizma buffered solutions may be autoclaved.  

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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