LC/MS/MS Analysis of Polymeric Isocyanates as Their HDI-DBA and IPDI-DBA Derivatives on Ascentis^® Express C18

sample preparation

SPE (Solid Phase Extraction)

sample/matrix

air

adsorbent tube

ASSET EZ4-NCO Dry Sampler (5028-U)

extraction

Contact Sigma-Aldrich Technical Services for extraction and full analytical protocol

column

Ascentis Express C18, 5 cm x 2.1 mm I.D., 2 μm particles (50811-U)

mobile phase

[A] 95:5 (0.05% formic acid in water):(0.05% formic acid in acetonitrile); [B] 95:5 (0.05% formic acid in acetonitrile):(0.05% formic acid in water

gradient

60 to 80% B in 2 min, hold for 3 min, to 100% B in 0.1 min, hold for 9.9 min, to 60% B in 0.1 min, hold for 3.9 mi

flow rate

0.4 mL/min

pressure

3626 psi (250 bar)

column temp.

35 °C

detector

MS/MS, ESI(+), MRM

injection

2.0 μL