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[主页](https://www.sigmaaldrich.com/SG/zh)[基因表达与沉默](https://www.sigmaaldrich.com/SG/zh/applications/genomics/gene-expression-and-silencing)载体图谱
# 载体图谱
MISSION 研发团队与[RNAi 联盟](https://portals.broadinstitute.org/gpp/trc1/user/login) (TRC)科学家合作，创造了一系列有助于成功开展RNAi实验的载体。基础载体 pLKO.1-puro 由 Broad Institute（TRC一员）开发。我们对 TRC 的贡献是多种用于关键对照实验的载体，以及用于定制 shRNA 生产的载体。本页面包含这类载体相关的信息。
[pLKO.1-puro 序列（无 shRNA 插入）](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/plko.txt)
[TTRC1.5 载体：pLKO.1-puro](https://www.sigmaaldrich.com#pLKO "TRC1.5 载体：pLKO.1-puro")
[TRC1.5 对照载体](https://www.sigmaaldrich.com#controlvectors "对照载体")
[TRC1.5 备用载体骨架](https://www.sigmaaldrich.com#alternate_vector "备用载体骨架")
[TRC2 载体：TRC2-pLKO-puro](https://www.sigmaaldrich.com#TRC2 "TRC2载体：TRC2-pLKO-puro")
[诱导型 shRNA 载体](https://www.sigmaaldrich.com#inducible "诱导型 shRNA 载体")
[慢病毒包膜载体](https://www.sigmaaldrich.com#lentiviral "慢病毒包膜载体")
[参考文献](https://www.sigmaaldrich.com#References "参考文献")
## [](https://www.sigmaaldrich.com)TRC1.5载体：pLKO.1-puro载体和应用
#### TRC1.5 克隆与 TRC1 克隆具有完全相同的载体骨架
pLKO.1-puro 载体的特征支持瞬时或稳定转染 shRNA 以及产生慢病毒颗粒。1使用嘌呤霉素筛选标记筛选稳定的基因沉默，同时通过与兼容的包膜质粒共转染，可在包膜细胞(HEK293T)中生成自失活的不可复制病毒颗粒。2,3作为我们产品组合独有的产品，TRC1.5 包含近 200,000 个克隆——包括逾49,000 个经过验证的克隆。它包含 TRC1 的所有内容，以及针对 2,661 个新人类基因和 2,395 个小鼠基因的另外 39,212 个克隆。与腺病毒或鼠类 MMLV 或 MSCV 逆转录病毒系统不同，慢病毒颗粒可有效感染特定 shRNA 构建体并掺入分化和非分裂细胞（例如神经元和树突细胞）中，克服了使用这些细胞系时的低转染和掺入困难问题。相对于siRNA和其他基于载体的系统，pLKO.1-puro为长期敲低和表型观测、复杂或敏感细胞系（非分裂细胞或原代细胞）转导提供了解决方案，且是一种经济的可再生资源。
pLKO.1-puro 质粒插入 shRNA 后，长度为 7,086 bp，如下方载体图谱所示。无 shRNA 插入片段时，pLKO.1-puro 载体长度为 7,052 bp。此处提供的序列档案是无插入片段的 pLKO.1-puro 载体（7,052 bp）。
[](https://www.sigmaaldrich.com)__TRC1.5载体图谱 (pLKO.1-puro)__
## TRC1.5 载体描述及特征
| | |
|---------|-------------------|
| 名称 | 描述 |
| cppt | 中央多聚嘌呤区 |
| hPGK | 人磷酸甘油酸激酶真核启动子 |
| puroR | 用于哺乳动物筛选的嘌呤霉素耐受基因 |
| SIN/LTR | 3' 自失活长末端重复序列 |
| f1 ori | f1 复制起点 |
| ampR | 用于细菌筛选的氨苄青霉素耐受基因 |
| pUC ori | pUC复制起点 |
| 5' LTR | 5'长末端重复序列 |
| Psi | RNA包膜信号 |
| RRE | Rev响应元件 |
## [](https://www.sigmaaldrich.com)TRC1.5对照载体
我们提供各种对照，助您开展最严格、详实的 shRNA 实验。请访问 shRNA 对照页面了解更多信息
| | | | |
|----------------------------------------------------------------------------|-------------------|------------------------------------------------------------------------------------------------------------------------------|--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|
| 产品 | 用途 | 载体图谱 | 载体档案 |
| [SHC001](https://www.sigmaaldrich.com/SG/zh/product/SIGMA/SHC001 "SHC001") | 空载体（无shRNA插入片段）对照 | [SHC001](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/204/376/shc001.pdf "SHC001 载体图谱 pdf") | [SHC001](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/SHC001.txt "SHC001载体档案") |
| [SHC002](https://www.sigmaaldrich.com "SHC002") | 非哺乳动物shRNA对照 | [SHC002](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/355/143/shc002.pdf "SHC002 载体图谱 pdf") | [SHC002](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/SHC002.txt) |
| [SHC003](https://www.sigmaaldrich.com/SG/zh/product/SIGMA/SHC003 "SHC003") | TurboGFP对照 | [SHC003](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/169/011/shc003.pdf "SHC003 载体图谱 pdf") | [SHC003](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/SHC003.txt "SHC003 载体档案") |
| [SHC004](https://www.sigmaaldrich.com/SG/zh/product/SIGMA/SHC004 "SHC004") | TurboGFP shRNA 对照 | [SHC004](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/419/352/shc004.pdf "SHC004 载体图谱 pdf") | [SHC004](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/SHC004.txt "SHC004 载体档案") |
| [SHC005](https://www.sigmaaldrich.com/SG/zh/product/SIGMA/SHC005 "SHC005") | eGFP shRNA对照 | [SHC005](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/101/008/shc005.pdf "SHC005 载体图谱 pdf") | [SHC005](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/SHC005.txt) |
| [SHC007](https://www.sigmaaldrich.com/SG/zh/product/SIGMA/SHC007 "SHC007") | 荧光素酶 shRNA 对照 | [SHC007](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/160/363/shc007.pdf "SHC007 载体图谱 pdf") | [](https://www.sigmaaldrich.com/SG/zh/product/sigma/shc007 "SHC007 载体档案")[SHC007](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/SHC007.txt "SHC00 载体档案") |
| SHC008 | B2M shRNA对照 | SHC008 | SHC008 |
| [SHC009](https://www.sigmaaldrich.com/SG/zh/product/SIGMA/SHC009 "SHC009") | ARHGDIA shRNA对照 | [SHC009](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/141/592/shc009.pdf "SHC009 载体图谱 pdf") | [SHC009](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/246/863/SHC009.txt "SHC009 载体档案") |
| SHC010 | CMV-TagCFP™ 对照 | SHC010 | SHC010 |
| [SHC011](https://www.sigmaaldrich.com/SG/zh/product/SIGMA/SHC011 "SHC011") | CMV-TagYFP™ 对照 | [SHC011](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/167/338/shc011.pdf "SHC011 载体图谱 pdf") | [SHC011](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc011-plko1-puro-cmv-tagyfp.txt "SHC011 pLKO.1-puro CMV-TagYFP 载体档案") |
| [SHC012](https://www.sigmaaldrich.com/SG/zh/product/SIGMA/SHC012 "SHC012") | CMV-TagRFP™ 对照 | [SHC012](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/370/026/SHC012.pdf "SHC012 载体图谱 pdf") | [SHC012](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc012-plko1-puro-cmv-tagrfp.txt "SHC012 pLKO.1-puro CMV-TagRFP 载体档案") |
| SHC013 | CMV-TagFP635™ 对照 | SHC013 | SHC013 |
| [SHC014](https://www.sigmaaldrich.com/SG/zh/product/SIGMA/SHC014 "SHC014") | UbC-TurboGFP™ 对照 | [SHC014](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/141/615/SHC014_v2.pdf "SHC014 载体图谱 pdf") | [SHC014](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc014-plko1-puro-ubc-turbogfp.txt "SHC014 pLKO.1-puro UbC-TurboGFP 载体档案") |
| [SHC015](https://www.sigmaaldrich.com/SG/zh/product/SIGMA/SHC015 "SHC015") | UbC-TagFP635™ 对照 | [SHC015](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/324/387/shc015.pdf) | [SHC015](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc013-plko1-puro-cmv-tagfp635.txt "SHC015 pLKO.1-puro UbC-TagFP635 载体档案") |
| [SHC016](https://www.sigmaaldrich.com/SG/zh/product/SIGMA/SHC016) | 非靶标shRNA对照 | [SHC016](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/259/151/shc016.pdf) | [SHC016](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc016-plko1-puro-non-target.txt) |
## TRC1.5 备用载体骨架
作为我们标准 shRNA 产品线的一部分，我们提供各种备用载体骨架。通过我们的自定义 MISSION shRNA 订购界面，可直接在质粒 DNA 或慢病毒转导颗粒克隆详情页面在线订购这类载体骨架的 TRC shRNA。如需定制发夹结构，请访问我们的定制克隆页面了解订购详情。
| | | | |
|----------------------|--------|------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|
| __产品__ | __用途__ | __载体图谱__ | __载体档案__ |
| pLKO.1-CMV-Neo | 备用载体骨架 | [pLKO.1-CMV-Neo](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/155/123/plko1-cmv-neo-shrna.pdf "pLKO.1-CMV-Neo 载体图谱 pdf") | [pLKO.1-CMV-Neo](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/plko1-cmv-neo-shrna.txt) |
| pLKO.1-Neo | 备用载体骨架 | [pLKO.1-Neo](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/319/429/plko1-neo-shrna.pdf "pLKO.1-hPGK-Neo 载体图谱 pdf") | [pLKO.1-Neo](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/plko1-cmv-neo-shrna.txt) |
| pLKO.1-CMV-tGFP | 备用载体骨架 | [pLKO.1-CMV-tGFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/153/942/plko1-cmv-tgfp.pdf "pLKO.1-CMV-tGFP 载体图谱 pdf") | [pLKO.1-CMV-tGFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/516/964/pLKO1-CMV-tGFP.txt "pLKO.1-CMV-tGFP 载体档案") |
| pLKO.1-Neo-CMV-tGFP | 备用载体骨架 | [pLKO.1-Neo-CMV-tGFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/108/350/plko1-hpgk-neo-cmv-tgfp.pdf "pLKO.1-hPGK-Neo-CMV-tGFP 载体图谱 pdf") | [pLKO.1-Neo-CMV-tGFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/plko1-neo-cmv-turbogfp-shrna.txt "pLKO.1-Neo-CMV-tGFP 载体档案") |
| pLKO.1-puro-CMV-tGFP | 备用载体骨架 | [pLKO.1-puro-CMV-tGFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/187/275/plko1-puro-cmv-tgfp.pdf "pLKO.1-puro-CMV-tGFP 载体图谱 pdf") | [pLKO.1-puro-CMV-tGFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/Plko1purocmvtgfp.txt "pLKO.1-puro-CMV-tGFP 载体档案") |
我们还提供多种含荧光蛋白或 UbC 启动子的备用载体骨架。这类构建体仅作为定制选项提供。请访问我们的自定义克隆页面，了解订购详情。
| | | | |
|---------------------------|--------|-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|
| 产品 | 用途 | 载体图谱 | 载体档案 |
| pLKO.1-puro-CMV-TagCFP™ | 备用载体骨架 | [pLKO.1-puro-CMV-TagCFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/308/460/plko1-puro-cmv-tagcfp.pdf) | [pLKO.1-puro-CMV-TagCFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc010-plko1-puro-cmv-tagcfp-shrna.txt "pLKO.1-puro-CMV-TagCFP载体档案") |
| pLKO.1-puro-CMV-TagYFP™ | 备用载体骨架 | [pLKO.1-puro-CMV-TagYFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/429/447/plko1-puro-cmv-tagyfp-shrna.pdf "pLKO.1-puro-CMV-TagYFP载体图谱 pdf") | [pLKO.1-puro-CMV-TagYFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc012-plko1-puro-cmv-tagrfp-shrna.txt "pLKO.1-puro-CMV-TagYFP载体档案") |
| pLKO.1-puro-CMV-TagRFP™ | 备用载体骨架 | [pLKO.1-puro-CMV-TagRFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/661/654/plko1-puro-cmv-tagrfp-shrna.pdf "pLKO.1-puro-CMV-TagRFP 载体图谱 pdf") | [pLKO.1-puro-CMV-TagRFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc012-plko1-puro-cmv-tagrfp-shrna.txt "pLKO.1-puro-CMV-TagRFP shRNA 载体档案") |
| pLKO.1-puro-CMV-TagFP635™ | 备用载体骨架 | [pLKO.1-puro-CMV-TagFP635](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/303/468/plko1-puro-cmv-tagfp635-shrna.pdf "pLKO.1-puro-CMV-TagFP635 载体图谱 pdf") | [pLKO.1-puro-CMV-TagFP635](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/726/928/shc013-plko1-puro-cmv-tagfp635-shrna.txt "pLKO.1-puro-CMV-TagFP635 shRNA 载体档案") |
| pLKO.1-puro-UbC-TurboGFP™ | 备用载体骨架 | [pLKO.1-puro-UbC-TurboGFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/405/968/plko1-puro-ubc-turbogfp.pdf) | [pLKO.1-puro-UbC-TurboGFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc014-plko1-puro-ubc-turbogfp-shrna.txt "pLKO.1-puro-UbC-TurboGFP shRNA 载体档案") |
| pLKO.1-puro-UbC-TagFP635™ | 备用载体骨架 | [pLKO.1-puro-UbC-TagFP635](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/324/387/shc015.pdf) | pLKO.1-puro-UbC-TagFP635 |
## TRC2载体：TRC2-pLKO-puro载体和应用
TRC2-pLKO-puro 载体还可用于瞬时或稳定转染 shRNA 以及慢病毒颗粒生产。TRC1.5 载体和 TRC2 载体之间的唯一区别是添加了 WPRE（土拨鼠肝炎病毒转录后调节元件）。4此元件可增强慢病毒递送的转基因表达。5与 TRC1.5 一样，TRC2 通过嘌呤霉素筛选标记实现稳定的基因沉默。利用表达载体与相容包装质粒共转染包装细胞(HEK293T)，可生产自灭活复制缺陷病毒颗粒。pLKO.1-puro为长期敲低和表型观测、复杂或敏感细胞系（非分裂细胞或原代细胞）转导以及增强转基因表达提供了解决方案。
插入 shRNA 后，TRC2-pLKO-puro 质粒长度为 7,518 bp，如下方载体图谱所示。无 shRNA 插入片段时，TRC2-pLKO-puro 载体长度为 7,484 bp。此处提供的序列档案是无插入片段的 TRC2-pLKO-puro 载体（7,484 bp）。
TRC2-pLKO-puro 序列（无 shRNA 插入）
__TRC2 载体图谱 (TRC2-pLKO-puro)__
| | |
|---------|-------------------|
| 名称 | 描述 |
| cppt | 中央多聚嘌呤区 |
| hPGK | 人磷酸甘油酸激酶真核启动子 |
| puroR | 用于哺乳动物筛选的嘌呤霉素耐受基因 |
| WPRE | 土拨鼠肝炎病毒转录后调节元件 |
| SIN/LTR | 3' 自失活长末端重复序列 |
| f1 ori | f1 复制起点 |
| ampR | 用于细菌筛选的氨苄青霉素耐受基因 |
| pUC ori | pUC复制起点 |
| 5' LTR | 5'长末端重复序列 |
| Psi | RNA包膜信号 |
| RRE | Rev响应元件 |
TRC2 载体描述及特征
| | | | |
|----------------------------------------------------------------------------|-------------------------|--------------------------------------------------------------------------------------------------------------------------------------|------------------------------------------------------------------------------------------------------------------------------------------------------------|
| __产品__ | __用途__ | __载体图谱__ | __载体档案__ |
| [SHC201](https://www.sigmaaldrich.com/SG/zh/product/sigma/SHC201 "SHC201") | 空 TRC2 载体（无shRNA插入片段）对照 | [SHC201](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/363/595/shc201_vector_map.pdf "SHC201 载体图谱 pdf") | [SHC201](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc201_plko-puro.txt "SHC201 载体档案") |
| [SHC202](https://www.sigmaaldrich.com/SG/zh/product/sigma/SHC202 "SHC202") | 非靶标shRNA对照 | [SHC202](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/150/531/shc202_vector_map.pdf "SHC202 载体图谱 pdf") | [SHC202](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc202_plko-puro_non-target_shrna.txt "SHC202 载体档案") |
| [SHC203](https://www.sigmaaldrich.com/SG/zh/product/sigma/SHC203 "SHC203") | TurboGFP对照 | [SHC203](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/305/613/shc203_vector_map.pdf "SHC203 载体图谱 pdf") | [SHC203](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc203_plko-puro_cmv-turbogfp.txt "SHC203 载体档案") |
| [SHC204](https://www.sigmaaldrich.com/SG/zh/product/sigma/SHC204 "SHC204") | TurboGFP shRNA 对照 | [SHC204](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/344/799/shc204_vector_map.pdf "SHC204 载体图谱 pdf") | [SHC204](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc204_plko-puro_shrna_to_turbogfp.txt "SHC204 载体档案") |
TRC2对照载体
请注意：目前，TRC2 暂无备用载体骨架。
### [](https://www.sigmaaldrich.com)诱导型 shRNA 载体
pLKO 载体经过重新设计，包含 LacI（阻遏蛋白）以及带有 LacO（操纵基因）序列的修饰人 U6 shRNA 启动子。无 IPTG（异丙基-β-D-硫代半乳糖苷，一种乳糖类似物）的情况下，LacI 可与 LacO 结合，阻止 shRNA 表达。存在 IPTG 时，变构 LacI 阻遏蛋白会改变构象，从 lacO 修饰的人 U6 启动子中释放，从而允许 shRNA 表达。
我们非常自豪可针对实际研究同时提供两种不同的 IPTG 诱导型载体。首选诱导型载体[pLKO-puro-IPTG-3xLacO](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/147/343/plko_iptg_3xlaco.pdf)包含三个 lac 操纵子序列（两个位于 U6 启动子中，一个位于启动子 3' 端），可提供严格的调控和良好的基因沉默效果。而 [pLKO-puro-IPTG-1xLacO](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/200/058/plko_iptg_1xlaco.pdf) 载体的 U6 启动子包含单个 lac 操纵子序列，有利于 shRNA 表达，但在未诱导时对启动子可控性较差。
| | | | |
|-----------------------|--------|----------------------------------------------------------------------------------------------------------------------------------------------------------------------------|------------------------------------------------------------------------------------------------------------------------------------------------------------------------|
| __产品__ | __用途__ | __载体图谱__ | __载体档案__ |
| pLKO-puro-IPTG-1xLacO | 备用载体骨架 | [pLKO-puro-IPTG-1xLacO](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/200/058/plko_iptg_1xlaco.pdf "pLKO-puro-IPTG-1xLacO 载体图谱 pdf") | [pLKO-puro-IPTG-1xLacO](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/plko-iptg-1xlaco.txt "pLKO-puro-IPTG-1xLacO 载体档案") |
| pLKO-puro-IPTG-3xLacO | 备用载体骨架 | [pLKO-puro-IPTG-3xLacO](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/147/343/plko_iptg_3xlaco.pdf "pLKO-puro-IPTG-3xLacO 载体图谱 pdf") | [pLKO-puro-IPTG-3xLacO](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/plko_iptg_3xlaco.txt "pLKO-puro-IPTG-3xLacO 载体档案") |
## [](https://www.sigmaaldrich.com)慢病毒包膜载体
最后，如客户希望自行包膜慢病毒，我们还可提供优化的包膜混合物。请访问包膜混合物页面了解更多详情。
__产品____用途____载体图谱____载体档案__
[SHP001](https://www.sigmaaldrich.com/SG/zh/product/sigma/shp001 "SHP001")
pMISSIONgagpol包膜载体 #1[pMISSIONgagpol](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/345/603/pmissiongagpol.pdf)
[pMISSIONgagpol](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/pMISSIONgagpol.txt)
[SHP001](https://www.sigmaaldrich.com/SG/zh/product/sigma/shp001 "SHP001")
pMISSIONvsvg包膜载体 #2[pMISSIONvsvg](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/307/260/pmissionvsvg.pdf "pMISSIONvsvg 载体图谱 pdf")
[pMISSIONvsvg](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/pcmv-vsv-g.txt)
### 参考文献
1\.
Stewart, S. A. et al. Lentivirus-delivered stable gene silencing by RNAi in primary cells. RNA 2003, 9, 493–501.. [https://doi.org/10.1261/rna.2192803](https://doi.org/10.1261/rna.2192803)
2\.
Zufferey, R. et al. Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo. Nat. Biotechnol 1997, 15, 871–85.. [https://doi.org/10.1038/nbt0997-871](https://doi.org/10.1038/nbt0997-871)
3\.
Zufferey, R. et al. Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery. J. Virol. 1998, 72, 9873–80.. [https://doi.org/10.1128/JVI.72.12.9873-9880.1998](https://doi.org/10.1128/JVI.72.12.9873-9880.1998)
4\.
Donello J. E. et al. Woodchuck hepatitis virus contains a tripartite posttranscriptional regulatory element. J. Virol. 1998, 72, 5085-92.. [https://doi.org/10.1128/JVI.72.6.5085-5092.1998](https://doi.org/10.1128/JVI.72.6.5085-5092.1998)
5\.
Zufferey, R. et al. Woodchuck hepatitis virus posttranscriptional regulatory element enhances expression of transgenes delivered by retroviral vectors. J. Virol. 1999, 73, 2886-92.. [https://doi.org/10.1128/JVI.73.4.2886-2892.1999](https://doi.org/10.1128/JVI.73.4.2886-2892.1999)
### 联系我们
如需了解有关图书馆、定价和报价或其他方面的问题，请通过电子邮件发给我们：[MISSIONRNAi@sial.com](mailto:MISSIONRNAi@sial.com).
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- [查看更多](https://www.sigmaaldrich.com/SG/zh/search/facet-search?focus=sitecontent&term=facet-search)
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