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Merck

UFC9010

Amicon® Ultra Centrifugal Filter, 10 kDa MWCO

greener alternative

sample volume 15 mL, regenerated cellulose membrane, MWCO 10 kDa

Synonym(s):

10 kDa MWCO, 15 mL sample volume, Centrifugal concentrator

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About This Item

UNSPSC Code:
41104916
eCl@ss:
36100101
NACRES:
NB.24

Key Documents

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material

polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing

Quality Level

400

sterility

non-sterile

product line

Amicon®

packaging

pkg of 24 pkg
pkg of 8 pkg
pkg of 96 pkg

manufacturer/tradename

Amicon® Ultra

sustainability

Greener Alternative Product

parameter

15 mL sample volume

technique(s)

protein extraction: suitable
protein purification: suitable

L

12.1 cm (4.807 in.)

diam.

2.97 cm

filtration area

7.6 cm2

volume

15 mL

working volume

≤15 mL
15 mL

pore size

10 kDa MWCO

greener alternative category

Re-engineered

shipped in

ambient

General description

Amicon® Ultra 15 mL centrifugal devices with Ultracel® 10,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, Cytochrome C (12.4 kDa) at 0.25 mg/ml initial concentration is retained at >95% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.

The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from 60% bio-circular material allocated using the ISCC mass balance approach. Click here for more information.

Application

  • Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel® membrane.
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
  • Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS.
  • Concentration of dilute or pre-purified proteins from column eluents.
  • Desalting, buffer exchange and protein dialysis.

Features and Benefits

  • Ultracel® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%.
  • Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10-15 minutes).
  • Heat-sealed membrane minimizes downstream extractables.
  • 100% integrity tested for reliable performance.
  • Convenient sample monitoring with translucent housing and volume gradations.
  • Direct pipettor sample access eliminates processing step to recover concentrate.
  • High concentration factors of 80–100X.

Physical form

Color Code: Clear with pink cap

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Protein Sample Ultrafiltration

Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.

Nanoparticle Ultrafiltration

Filtration methods and membrane filter selection in nanoparticle purification and production.

Protein Sample Ultrafiltration

สำรวจหลักการของเมมเบรนกรองและประโยชน์สำหรับการเตรียมตัวอย่างโปรตีนรวมถึงการแลกเปลี่ยนตัวทำละลายและความเข้มข้นของตัวอย่าง

อนุภาคนาโน Ultrafiltration

วิธีการกรองและการเลือกตัวกรองเมมเบรนในการทำให้บริสุทธิ์อนุภาคนาโนและการผลิต

Protocols

โปรโตคอลถุงนอกเซลล์

แนวทางปฏิบัติที่ดีที่สุดสำหรับการสร้างมาตรฐานและลดขั้นตอนการเตรียมถุงลมนอกเซลล์และขจัดการปนเปื้อนของการเตรียม EV ด้วยถุงลมที่ได้จากเซลล์ตายแล้ว

Extracellular Vesicle Protocol

Best practices for standardizing and reducing the Extracellular Vesicle Preparation workflow, and eliminating contamination of EV preparations with dead-cell-derived vesicles.

Related Content

Amicon® Ultra Centrifugal Filters

โปรตีนเข้มข้นไม่สูญเสียมัน ฟื้นฟูโปรตีนได้อย่างน่าเชื่อถือเมื่อคุณมีสมาธิด้วยตัวกรอง Amicon ® Ultra

User Guide-Amicon® Ultra-15 Centrifugal Filter Devices

Questions

1–8 of 8 Questions  

  1. Is there a user manual anywhere? What is the recommended g force to be used with this filter?

    1 answer

    1. The spin conditions for a swinging-bucker rotor are 4000 x g and for a fixed-angle rotor are 5000 x g, at a time range of 10 - 60 minutes, based on molecular weight cut-off.

      Please see the link below to review the Product User Guide:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/317/181/pr05035wrev1023-ms.pdf

      Helpful?

  2. Is there a recommended temperature for the amicon ultra-15? I would like to concentrate protein at 0 degrees C. Also, is glycerol able to pass through the 30kD filter? The enzyme I want to concentrate is in 50% glycerol. Thanks!

    1 answer

    1. No data is available on the maximum or minimum temperature for using the Amicon Ultra-15. Results shown in the user guides are based on experiments conducted at approximately 25°C, not with refrigerated centrifuges. Some users work at 4°C, but filtration times are longer. No studies have been performed at 0°C. Glycerol is compatible with this product.

      Helpful?

  3. Can I concentrate cell-propagated respiratory syncytial virus stock using the Amicon® Ultra Centrifugal Filter, 10 kDa MWCO? what is your though and suggestion?

    1 answer

    1. Concentrating cell-propagated respiratory syncytial virus stock with this product has not been studied. However, it can be attempted, as other viruses such as SARS, AAV, and Lenti have been successfully concentrated. The product concentrates substances larger than the molecular weight cut-off of the membrane. It is recommended to consult online research articles using keywords like "Amicon and respiratory syncytial virus" on platforms such as PubMed or Google Scholar.

      Helpful?

  4. Do you make a miniature version of the Amicon® Ultra Centrifugal Filter for 1.5ml samples?

    1 answer

    1. Yes, product UFC5010, the Amicon Ultra 2.0 for samples of 0.5 - 2.0 mL, will accommodate a 1.5 mL sample size. Please see the link below to review this product option:
      https://www.sigmaaldrich.com/product/mm/ufc5010

      Helpful?

  5. Does ultrafiltration of protein samples result in lower migration on an SDS-PAGE gel?

    1 answer

    1. ​Yes, ultrafiltration of protein samples can potentially lead to lower migration on an SDS-PAGE gel.During the ultrafiltration process, the desalting or buffer exchange step may remove or reduce sodium chloride (NaCl), leading to lower protein migration on the gel. To prepare protein samples for gel electrophoresis, using an Amicon Ultra (AU) device or a suitable desalting/buffer exchange method is recommended.

      Helpful?

  6. Hello, How much filters represent 1pkg? Only one filter? A full pack? Thank you,

    1 answer

    1. Amicon® Ultra-15 Centrifugal Filter Unit comes in 8, 24, and 96/pk. the pack size is indicated in the last two digits of the catalog numbers.

      Helpful?

  7. Hello, I'm using UFC903096 (Amicon-15, 30K) and am trying to exchange and concentrate without over-concentrating <500uL ... Would you have a table of the volume to add to the filtrate collector to ensure ~ final product volumes. Thanks!

    1 answer

    1. This product is suitable for sample volumes of less than 15 mL. Please see the link below to review the estimated volumes:
      https://www.sigmaaldrich.com/products/protein-biology/protein-sample-prep/amicon-centrifugals#howtoselect

      Helpful?

  8. How to wash the column after one time used for the buffer exchange or for protein concentration. Is any protocol please share??

    1 answer

    1. We do not recommend reusing the device because eventually the membrane will clog completely. We have yet to record an efficient way to clean the pores of the membrane without damaging the membrane itself.

      Helpful?

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