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UFC5010

Amicon® Ultra Centrifugal Filter, 10 kDa MWCO

sample volume 0.5 mL, regenerated cellulose membrane, MWCO 10 kDa

Sustainability features

1 of 2 reviewers took part in a promotion

Synonym(s):

Centrifugal concentrator, 10 kDa MWCO, 0.5 mL sample volume

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Size/SKUAvailabilityPrice
24 pkg

Estimated to ship onNovember 23, 2026

$201.00
96 pkg

Estimated to ship onNovember 23, 2026

$632.00
500 pkg

Estimated to ship onDecember 07, 2026

$2,580.00

About This Item

UNSPSC Code:
41104916
NACRES:
NB.24
eCl@ss:
36100101
Material:
polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing
Pore size:
10 kDa MWCO
Working volume:
≤500 μL , 0.5 mL
Sterility:
non-sterile
Packaging:
pkg of 24 pkg, pkg of 500 pkg, pkg of 8 pkg, pkg of 96 pkg

$201.00

Estimated to ship onNovember 23, 2026Details

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Quality Segment

material

polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing

sterility

non-sterile

product line

Amicon®

packaging

pkg of 24 pkg, pkg of 500 pkg, pkg of 8 pkg, pkg of 96 pkg

manufacturer/tradename

Amicon® Ultra

sustainability

Sustainability features

parameter

0.5 mL sample volume

technique(s)

Diafiltration: suitable, protein extraction: suitable, protein purification: suitable

L

4.99 cm (1.96 in.)

diam.

10.8 mm

filtration area

1 cm2

volume

0.5 mL

working volume

≤500 μL , 0.5 mL

pore size

10 kDa MWCO

greener alternative category

shipped in

ambient

General description

The inner filtration device is made from bio-circular material allocated using the ISCC mass balance approach. Click here for more information.
Amicon® Ultra-0.5 centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, Cytochrome c (12.4 kDa) at 0.25 mg/ml initial concentration is typically retained at >95% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.

Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.

Application

  • Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel® membrane
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
  • Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
  • Desalting, buffer exchange, and protein dialysis

Features and Benefits

  • Ultracel® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%
  • Concentrates 500 μL down to 15 μL
  • Concentration factor 25x to 30x
  • High sample recovery (greater than 90% of dilute starting solution)
  • Fast processing time: 10 to 30 minutes
  • Reverse spin capability provides consistent recoveries

Physical form

Color Code: Clear

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

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Amicon® Ultra Centrifugal Filter, 10 kDa MWCO sample volume 0.5 mL, regenerated cellulose membrane, MWCO 10 kDa

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UFC9010

Amicon® Ultra Centrifugal Filter, 10 kDa MWCO

description

sample volume 0.5 mL, regenerated cellulose membrane, MWCO 10 kDa

description

sample volume 2 mL, regenerated cellulose membrane, MWCO 10 kDa

description

sample volume 4 mL, regenerated cellulose membrane, MWCO 10 kDa

description

sample volume 15 mL, regenerated cellulose membrane, MWCO 10 kDa

manufacturer/tradename

Amicon® Ultra

manufacturer/tradename

Amicon® Ultra

manufacturer/tradename

Amicon® Ultra

manufacturer/tradename

Amicon® Ultra

pore size

10 kDa MWCO

pore size

10 kDa MWCO

pore size

10 kDa MWCO

pore size

10 kDa MWCO

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

parameter

0.5 mL sample volume

parameter

2 mL sample volume

parameter

4 mL sample volume

parameter

15 mL sample volume

material

styrene-butadiene housing, regenerated cellulose membrane, polypropylene tube, polypropylene cap

material

styrene-butadiene housing, polypropylene tube (for collection), regenerated cellulose membrane

material

polypropylene tube, polyethylene cap, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing, polyethylene liner

material

polypropylene tube, styrene-butadiene housing, regenerated cellulose support, polyethylene cap, polyethylene liner, regenerated cellulose membrane

working volume

0.5 mL , ≤500 μL

working volume

≤2 mL

working volume

≤4 mL

working volume

≤15 mL

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Questions

1–10 of 11 Questions  

  1. How do I reverse spin the concentrated sample?

    1 answer

    1. The reverse spin process requires inversion of the ultrafiltration device into an appropriate collection tube in order to maximize sample recovery. To recover the concentrated solute, place the Amicon® Ultra filter device upside down in a clean microcentrifuge tube. Place in centrifuge, aligning open cap towards the center of the rotor; counterbalance with a similar device. Spin for 2 minutes at 1,000 × g to transfer the concentrated sample from the device to the tube. The ultrafiltrate can be stored in the centrifuge tube. Please see the link below to view and instructional video:
      https://www.youtube.com/watch?v=jXnKT6xHY-8

      Please see the link below to review additional details and instructions in the product User Guide:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/105/563/pr05485w-ug-ms.pdf

      The following link includes additional helpful information regarding the selection and use of Amicon UltraFiltration Devices:
      https://www.sigmaaldrich.com/products/protein-biology/protein-sample-prep/amicon-centrifugals#ultracentrifugal

      Helpful?

  2. Can the polypropylene collection tubes be autoclaved prior to the filtration? And is there a way to sterilize the filter e.g. with 70% ethanol?

    1 answer

    1. These tubes should not be autoclaved prior to use, as we have not studied this for integrity.  Tubes can be sanitized with 70% ethanol.  However, the ethanol only sanitizes the device; it does not sterilize it.  

      Helpful?

  3. Can I purchase just the flow through columns?

    1 answer

    1. The Amicon Ultra products come in either 2 piece or 3 piece configurations. It is not possible to order just the filter inner tube or the empty outer tube. These two pieces fit together and are sold together as they are always needed. One sample, one centrifugal product. With the Amicon Ultra 0.5 or 2, we have a third piece, an outer collection tube added which is optionally used for reverse spin capability. Please consult our User Guide.  

      Helpful?

  4. What is the minimum volume allowed by the device? If we have a volume of 50 microliters, is it advisable to dilute it in PBS to obtain a larger volume, closer to 500 microliters?

    1 answer

    1. Diluting to 500 ul is optional. You have to consider what level of concentration is expected. Amicons can concentrate down to about 15 ul, so a 50 ul sample can only be concentrated ~3.3 fold.

      Helpful?

  5. What is the pore diameter of Amicon® Ultra Centrifugal Filter, 10 kDa

    1 answer

    1. The ultrafiltration membrane in this product is characterized by a nominal molecular weight limit based on protein retention. An absolute linear pore diameter is not determined. However, a membrane with a 10 kDa molecular weight cutoff will retain nanoparticles of 3-9 nanometers. Please see the related article on nanoparticle ultrafiltration: https://www.sigmaaldrich.com/technical-documents/technical-article/analytical-chemistry/filtration/nanoparticle-ultrafiltration

      Helpful?

  6. What is the tolerance for molecular weight with these spin columns? Could you reasonably purify a protein of 15 kDa using these columns, or are the tolerances too high and would result in loss of protein into the filtrate?

    1 answer

    1. It is recommended to choose a molecular weight cut-off that is at least two times smaller than the molecular weight of the target molecules.

      Helpful?

  7. Is this filter suitable for purifying MHC-bound peptides as filtrate/eluate while trapping proteins and antibodies as retentate following Immunoprecipitation?

    1 answer

    1. This product is not suitable for separating protein/peptides of different sizes.

      Helpful?

  8. If a centrifuge is unable to achieve a spinning speed of 14000 X g as recommended in the protocol, is it possible to compensate by spinning the samples for a longer duration?

    1 answer

    1. Yes, it is possible to compensate for a lower centrifuge spinning speed by increasing the duration. This can be achieved by calculating the K factor, which determines the adjustment needed in time.

      Helpful?

  9. se puede utilizar los UFC501024 para concentrar una proteina glicosilada?

    1 answer

    1. Yes, Amicon devices like UFC501024 can be used to concentrate any protein greater than about 20 kDa.

      Helpful?

  10. Dear Sir/Mam, can you let me know how many tubes is 8 pkg? Is it 8 tubes? Best regards, Alejandra

    1 answer

    1. It is 8 tubes per pack.

      Helpful?

1–10 of 11 Questions  

Reviews

1 of 2 reviewers took part in a promotion

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  1. 2 Ratings-Only Reviews