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Merck

P0380

L-Proline

≥99% (HPLC), ReagentPlus®

Synonym(s):

(S)-Pyrrolidine-2-carboxylic acid

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About This Item

Empirical Formula (Hill Notation):
C5H9NO2
CAS Number:
Molecular Weight:
115.13
Beilstein:
80810
EC Number:
MDL number:
UNSPSC Code:
12352209
eCl@ss:
32160406
PubChem Substance ID:
NACRES:
NA.26

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Product Name

L-Proline, ReagentPlus®, ≥99% (HPLC)

Quality Level

product line

ReagentPlus®

Assay

≥99% (HPLC)

form

powder

impurities

L-Hydroxyproline, free

color

white

mp

228 °C (dec.) (lit.)

application(s)

detection

SMILES string

OC(=O)[C@@H]1CCCN1

InChI

1S/C5H9NO2/c7-5(8)4-2-1-3-6-4/h4,6H,1-3H2,(H,7,8)/t4-/m0/s1

InChI key

ONIBWKKTOPOVIA-BYPYZUCNSA-N

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1 of 4

This Item
P5607936931568506
form

powder

form

powder

form

-

form

-

assay

≥99% (HPLC)

assay

98.5-101.0% dry basis

assay

-

assay

-

product line

ReagentPlus®

product line

-

product line

TraceCERT®

product line

-

impurities

L-Hydroxyproline, free

impurities

endotoxin, tested

impurities

-

impurities

-

color

white

color

white

color

white

color

-

Quality Level

200

Quality Level

300

Quality Level

300

Quality Level

-

Application

L-Proline has been used:
  • As a supplement during the preparation of chondrogenic medium[1] and synthetic dextrose minimal medium (SD).[2]
  • As a standard during the identification of metabolites in serum samples.[3]
  • In a study to prepare L-proline-L-phenylalanine (L-Pro-L-Phe) mixture in aqueous acetonitrile.[4]

Legal Information

ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany

used together

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

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Andrew Davison
Eureka: Biochemistry & Metabolism, 2015, 76-76 (2015)
Proline: a multifunctional amino acid
La' szlo' Szabados and Arnould Savoure
Trends in Plant Science, 1-9 (2009)
Metabolomic insights into system-wide coordination of vertebrate metamorphosis.
Ichu TA, et al.
BMC Developmental Biology, 14(1), 1-1 (2014)
Mieczysław Sajewicz et al.
Journal of chromatographic science, 53(1), 31-37 (2014-03-05)
We employ the achiral liquid chromatography with diode array, evaporative light scattering and mass spectrometric detection (HPLC-DAD, HPLC-ELSD and LC-MS) to assess structural instability (understood as spontaneous oscillatory chiral conversion and spontaneous oscillatory condensation) of the two pairs of amino
Quantification of extracellular ammonium concentrations and transporter activity in yeast using AmTrac fluorescent sensors.
Ast C, et al.
Bio-protocol, 5, e1372-e1372 (2015)

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Amino Acid Codon Wheel for fast RNA translation. Find which amino acid is translated from your RNA sequence quickly and easily.

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Chromatograms

Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. The CoA says Purity by HPLC. How was this obtained? Type of chromatography, column, detection? Important information considering Proline's high polarity and lack of chromophores.

    1 answer
    1. Regrettably, the analytical methods for this product are considered proprietary. However, this material is analyzed by reverse phase HPLC. Samples are derivatized and detection is at 260 nm.

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