05-655

Anti-p21/WAF1/Cip1 Antibody, clone CP74

Name: Anti-p21/WAF1/Cip1 Antibody, clone CP74
Brand: MM

clone CP74, Upstate^®, from mouse

Synonym(s):

Anti-CAP20, Anti-CIP1, Anti-MDA-6, Anti-P21, Anti-SDI1, Anti-WAF1, Anti-p21CIP1

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

Key Documents

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biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

CP74, monoclonal

species reactivity

human

manufacturer/tradename

Upstate^®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NM_000389

UniProt accession no.

P38936

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CDKN1A(1026)

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This Item	05-345	SAB4504200	P1484
Sigma-Aldrich 05-655 Anti-p21/WAF1/Cip1 Antibody, clone CP74	Sigma-Aldrich 05-345 Anti-p21/WAF1/Cip1 Antibody	Sigma-Aldrich SAB4504200 Anti-phospho-p27 Kip1 (pThr¹⁹⁸) antibody produced in rabbit	Sigma-Aldrich P1484 Anti-p21^WAF1/Cip1antibody, Mouse monoclonal
biological source mouse	biological source mouse	biological source rabbit	biological source mouse
species reactivity human	species reactivity mouse, human	species reactivity human, mouse, rat	species reactivity mouse, human
antibody form purified antibody	antibody form purified immunoglobulin	antibody form affinity isolated antibody	antibody form purified immunoglobulin
clone CP74, monoclonal	clone CP36, monoclonal, CP74, monoclonal	clone polyclonal	clone CP74, monoclonal
UniProt accession no. P38936	UniProt accession no. P38936	UniProt accession no. P46527	UniProt accession no. P38936
shipped in wet ice	shipped in dry ice	shipped in wet ice	shipped in dry ice

General description

21kDa

Immunogen

Recombinant full-length human Cip1 with an N-terminal histidine tag; epitope corresponds to amino acids 1-80 of full-length human Cip1

Application

Anti-p21/WAF1/Cip1 Antibody, clone CP74 is a high quality Mouse Monoclonal Antibody for the detection of p21/WAF1/Cip1 & has been validated in WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Biochem/physiol Actions

Cip1

Physical form

0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%

Format: Purified

Protein G Purified

Preparation Note

2 years at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

routinely evaluated by immunoblot on nuclear extract from HeLa cells

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pricing

ZRB1141

Anti-p21 Antibody, clone 5G7, ZooMAb^® Rabbit Monoclonal, recombinant, expressed in HEK 293 cells

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Activating mutations in CTNNB1 in aldosterone producing adenomas.

Tobias Åkerström et al.

Scientific reports, 6, 19546-19546 (2016-01-28)

Primary aldosteronism (PA) is the most common cause of secondary hypertension with a prevalence of 5-10% in unreferred hypertensive patients. Aldosterone producing adenomas (APAs) constitute a large proportion of PA cases and represent a surgically correctable form of the disease.

Heat shock protein 90 inhibitors attenuate LPS-induced endothelial hyperpermeability.

Chatterjee, A; Snead, C; Yetik-Anacak, G; Antonova, G; Zeng, J; Catravas, JD

American Journal of Physiology. Lung Cellular and Molecular Physiology null

Recessive Mutation in FAM83G Associated with Palmoplantar Keratoderma and Exuberant Scalp Hair.

Thiviyani Maruthappu et al.

The Journal of investigative dermatology, 138(4), 984-987 (2017-11-16)

Evidence for different modes of action of cyclin-dependent kinase inhibitors: p15 and p16 bind to kinases, p21 and p27 bind to cyclins.

M Hall et al.

Oncogene, 11(8), 1581-1588 (1995-10-19)

A number of low molecular weight proteins have recently been identified that specifically inhibit the function of cyclin-dependent protein kinases in mammalian cells. These fall into two distinct families based on primary sequence comparisons and probable modes of action. Using

Expression and function of EZH2 in synovial fibroblasts: epigenetic repression of the Wnt inhibitor SFRP1 in rheumatoid arthritis.

Trenkmann M, Brock M, Gay RE, Kolling C, Speich R, Michel BA, Gay S, Huber LC

Annals of the Rheumatic Diseases null

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