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23-024-M

p27 Protein complex, activated, 10 µg

Activated complex of three (3) full length, recombinant human proteins: p27, Cyclin E1, & CDK2. Phosphorylated in vitro using CDK2/Cyclin E1. For use in Enzyme Assays. Behaves as a substrate in ubiquitin assays.

Synonym(s):

p27 kip1, Cyclin-dependent kinase inhibitor 1B, Kip1

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.41

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biological source

human

Quality Level

100

recombinant

expressed in baculovirus infected Sf21 cells

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable

GenBank accession no.

BC001971 (p27)

NCBI accession no.

NM_001238 (Cyclin E1)
NM_001798

Gene Information

human ... CDK2(1017), CDKN1B(1027)

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This Item
23-023-M23-030-MSAB5700634
p27 Protein complex, activated, 10 µg Activated complex of three (3) full length, recombinant human proteins: p27, Cyclin E1, & CDK2. Phosphorylated in vitro using CDK2/Cyclin E1. For use in Enzyme Assays. Behaves as a substrate in ubiquitin assays.

Sigma-Aldrich

23-024-M

p27 Protein complex, activated, 10 µg

SCF-Skp2 Protein Complex, Active, 10 µg Active complex of five (5) full length, recombinant human enzymes: Skp1, Skp2, Cul1, Rbx1 & Cks1. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.

Sigma-Aldrich

23-023-M

SCF-Skp2 Protein Complex, Active, 10 µg

SCF-Fbw7 Protein Complex, Active, 10 µg Active complex of four (4) full length, recombinant human enzymes: Fbw7, Skp1, Cul1 & Rbx1. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.

Sigma-Aldrich

23-030-M

SCF-Fbw7 Protein Complex, Active, 10 µg

Anti-CDKN1B antibody produced in rabbit

Sigma-Aldrich

SAB5700634

Anti-CDKN1B antibody produced in rabbit

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

-

biological source

human

biological source

human

biological source

human

biological source

rabbit

technique(s)

activity assay: suitable

technique(s)

activity assay: suitable

technique(s)

activity assay: suitable

technique(s)

immunofluorescence: 1:20-1:50, immunohistochemistry: 1:50-1:200, western blot: 1:500-1:2000

Gene Information

human ... CDK2(1017), CDKN1B(1027)

Gene Information

human ... Cks1(1163), Cul1(8454), Rbx1(9978), Skp1(6500), Skp2(6502)

Gene Information

human ... Cul1(8454), Fbw7(55294), Rbx1(9978), Skp1(6500)

Gene Information

human ... CDKN1B(1027)

NCBI accession no.

NM_001238 (Cyclin E1), NM_001798

NCBI accession no.

NM_001826, NM_003592, NM_005983, NM_014248, NM_170679

NCBI accession no.

NM_003592, NM_014248, NM_033632, NM_170679

NCBI accession no.

-

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

General description

p27 is an important regulator of cell cycle progression, involved in G1 arrest. It is a potent inhibitor of cyclin E1- and cyclin A-CDK2 complexes and a positive regulator of cyclin D-dependent kinases such as CDK4. The degradation of this protein, which is triggered by its CDK-dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Decreased levels of p27 are found in various epithelial tumours originating from lung, breast, colon, ovary, oesophagus, thyroid and prostate. Defects in the gene encoding p27 (CDKN1B) are the cause of multiple endocrine neoplasia type 4 (MEN4) an inherited cancer of the thyroid.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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