AP180P

Donkey Anti-Goat IgG Antibody, HRP conjugate, Species Adsorbed

Chemicon^®, from donkey

• Synonym(s): Anti-Rabbit IgG HRP, HRP Donkey Anti-Rabbit IgG
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.46

Properties

• biological source: donkey
• Quality Level: 100
• conjugate: peroxidase conjugate
• antibody form: purified immunoglobulin
• antibody product type: secondary antibodies
• clone: polyclonal
• species reactivity: goat
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• shipped in: wet ice
• target post-translational modification: unmodified

Description

General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Application

Donkey anti-Goat IgG Antibody, HRP conjugate, Species Adsorbed is an antibody against Donkey Goat IgG for use in ELISA, IH & WB.

Immunohistochemistry:
1:500-1:5,000 dilution can be used.

Optimal working dilutions must be determined by end user.

Physical form

Purified donkey secondary antibody IgG in lyophilized buffer 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA.
Reconstitution:
Reconstitute with 500 μL of sterile distilled water.

Preparation Note

Maintain lyophilized product at 2-8°C for up to 12 months.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.

Analysis Note

Routinely tested on Enzyme-linked Immunosorbent Assay.

ELISA and Western Blots:
1:5,000-1:100,000 dilution can be used.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3