The speed needs to be evaluated. However, we know that the maximum speed should never be exceeded. It is best to run below the maximum speed to not risk breakage in case the centrifuge has not been calibrated recently. Here are our specifications for the Amicon Ultra 0.2: Maximum relative centrifugal force Swinging bucket rotor 4,000 × g for concentration spin, 1,000 × g for recovery spin Fixed angle rotor 7,500 × g for concentration spin, 1,000 × g for recovery spin
NOTE: When spinning viscous solutions such as undiluted serum or plasma, do not exceed 5,400 x g. Furthermore, if you are trying to concentrate proteins, often, proteins do not like to be spun at a high speed or they can precipitate. Therefore, let's say your centrifuge is run at 2000 x g. This should work fine. It may take longer or it may not. This depends on initial and final desired volume when stopped.
UFC201024
Amicon® Ultra Centrifugal Filter, 10 kDa MWCO
sample volume 2 mL, regenerated cellulose membrane, MWCO 10 kDa
Synonym(s):
Centrifugal concentrator, 10 kDa MWCO, 2 mL sample volume
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About This Item
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material
polypropylene tube (for collection)
regenerated cellulose membrane
styrene-butadiene housing
Quality Level
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Greener Alternative Product
parameter
2 mL sample volume
technique(s)
protein extraction: suitable
protein purification: suitable
L
119.7 mm
diam.
15.9 mm
filtration area
1 cm2
volume
2 mL
working volume
≤2 mL
2 mL
pore size
10 kDa MWCO
greener alternative category
shipped in
ambient
1 of 4
This Item | UFC8010 | UFC5010 | UFC9010 |
|---|---|---|---|
| pore size 10 kDa MWCO | pore size 10 kDa MWCO | pore size 10 kDa MWCO | pore size 10 kDa MWCO |
| parameter 2 mL sample volume | parameter 4 mL sample volume | parameter 0.5 mL sample volume | parameter 15 mL sample volume |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra |
| material polypropylene tube (for collection), styrene-butadiene housing, regenerated cellulose membrane | material polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing | material polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing | material polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing |
| product line Amicon® | product line Amicon® | product line Amicon® | product line Amicon® |
General description
Application
- Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates.
- Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC.
- Desalting, buffer exchange and protein dialysis.
- Size exclusion and protein fractionation.
- to reduce large volume biofluids prior to extracellular vesicle (EV) isolation or to concentrate EVs[1]
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids (DNA/RNA samples, either single- or double-stranded), microorganisms, column eluates, and purified samples
- Purification of macromolecular components found in tissue culture extracts and cell lysates, removal of primer, linkers, or molecular labels from a reaction mix, and protein removal before high-performance liquid chromatography (HPLC)
- Desalting, buffer exchange[2], or diafiltration
Features and Benefits
- Amicon® Ultra 2ml 10K 24PK
- Concentrates 2 mL down to 15-70 μl
- 98% sample recovery
- Fast processing time - 10 to 30 minute spin times
- Engineered dead stop
- The reverse spin capability provides consistent recoveries
- Ultracel® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%
- Concentrates 2 mL down to 15-70 μL
- 98% sample recovery
- Fast processing time: 10 to 30-minute spin times
- Engineered dead stop
- Reverse spin capability provides consistent recoveries
Other Notes
Legal Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
探索超濾膜在蛋白質樣品製備方面的原理和優點，包括溶劑交換和樣品濃縮。
Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.
Protocols
標準化和減少細胞外小泡製備工作流程的最佳實踐，消除死細胞衍生小泡對 EV 製備的污染。
Best practices for standardizing and reducing the Extracellular Vesicle Preparation workflow, and eliminating contamination of EV preparations with dead-cell-derived vesicles.
Related Content
濃縮蛋白質，避免流失。使用 Amicon® Ultra 過濾器進行濃縮時，能可靠地回收蛋白質。
"The Amicon® Ultra 2 mL filter provides a fast and easy way to concentrate, desalt and exchange thebuffer for both proteins and nucleic acids. Now you can concentrate 2 mL down to 15 – 70 μL in anAmicon® Ultra centrifugal device."
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At what speed should the Amicon® Ultra Centrifugal Filter, 10 kDa MWCO UFC201024 be used? Besides, what is the maximun g-force supported by the Amicon?
1 answer-
Helpful?
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Can Amicon® Ultra Centrifugal Filter, 10 kDa MWCO UFC201024 to be in the Centrifuge as 4 mL Centrifugal Filter without adaptors？We have adaptors for 15 mL TUBES
1 answer-
Amicon Ultra-2 devices should be used in a centrifuge with swinging bucket or fixed angle rotor with wells/carriers that can accommodate 17 mm × 100 mm tubes. This is the same well/carrier size that is recommended for the Amicon® Ultra-4 devices. To avoid damage to the device during centrifugation, make sure it is properly assembled and seated at the bottom of the rotor. The rim of the concentrate collection tube should be inside the rotor well. Check clearance before spinning.
Helpful?
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Hello. Does these 2 ml 10 kDa filters fit into the same centrifuge rotor as the 0.5 ml 10 kDa amicon filters?
1 answer-
No, the Amicon-0.5 devices require a microfuge, while the Amicon-2 devices require a centrifuge with adapters for 5 ml tubes.
Helpful?
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