71736

Sodium dodecyl sulfate solution

BioUltra, Molecular Biology, 10% in H₂O

• Synonym(s): Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate solution
• Linear Formula: CH₃(CH₂)₁₁OSO₃Na
• CAS Number: 151-21-3
• Molecular Weight: 288.38
• Beilstein: 3599286
• MDL number: MFCD00036175
• UNSPSC Code: 12161902
• eCl@ss: 39093306
• PubChem Substance ID: 57652355
• NACRES: NA.26

Properties

• grade: Molecular Biology
• Quality Level: 200
• description: anionic
• product line: BioUltra
• form: liquid
• mol wt: 288.38 g/mol
• greener alternative product characteristics: Catalysis; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• concentration: 10% in H₂O
• technique(s): HPLC: suitable; protein quantification: suitable
• impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• pH: 5.0-6.5
• density: 1.01 g/mL at 20 °C
• anion traces: chloride (Cl^-): ≤50 mg/kg; phosphate (PO₄^3-): ≤1 mg/kg
• cation traces: Al: ≤1 mg/kg; As: ≤0.1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Zn: ≤1 mg/kg
• λ: neat
• UV absorption: λ: 260 nm A_max: 0.3; λ: 280 nm A_max: 0.2
• SMILES string: [Na+].CCCCCCCCCCCCOS([O-])(=O)=O
• application(s): (Western Blot)
• greener alternative category: Aligned
• InChI: 1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1
• InChI key: DBMJMQXJHONAFJ-UHFFFAOYSA-M

Description

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is used as a surfactant to enhance catalysis.  Click here for more information.

Application

Sodium dodecyl sulfate solution has been used:

• in the preparation of 2x extraction buffer[1]
• as a component in elution buffer for immunoprecipitation[2]
• in SDS–polyacrylamide gel electrophoresis (PAGE) analysis[3][4]

Biochem/physiol Actions

Sodium dodecyl sulfate (SDS)/lauryl sulfate, is an ionic detergent has the ability to disrupt biological membranes. It can block ribonuclease (RNase) and deoxyribonuclease (DNase) activity. SDS is one of the main constituents of several reagents, that are used to purify nucleic acids.[5] It is also considered as a permeability enhancer in oral formulations.[6]

Other Notes

In large-scale phenol extraction of RNA - promotes the dissociation of protein from nucleic acids when extracting from biological material[7]

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H315,H319
• Precautionary Statements: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves