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Merck

B4287

Bovine Serum Albumin

heat shock fraction, protease free, suitable for hybridization, pH 7, ≥98%

Synonym(s):

Albumin bovine serum, BSA, Bovine albumin

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About This Item

CAS Number:
9048-46-8
EC Number:
232-936-2
MDL number:
MFCD00130384
UNSPSC Code:
12352202
NACRES:
NA.25

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biological source

bovine

grade

Molecular Biology

product line

BioReagent

Assay

≥98%

form

lyophilized powder

mol wt

~66 kDa

purified by

heat shock fractionation

packaging

poly bottle of

origin

USA origin

technique(s)

ELISA: suitable
electrophoresis: suitable
western blot: suitable

impurities

protease, free

pH

7

solubility

water: soluble (40 mg/ml)

UniProt accession no.

P02769

foreign activity

Protease free

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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1 of 4

This Item
A3059A7030A3803
Bovine Serum Albumin heat shock fraction, protease free, suitable for hybridization, pH 7, ≥98%

Sigma-Aldrich

B4287

Bovine Serum Albumin

Bovine Serum Albumin heat shock fraction, protease free, essentially globulin free, pH 7, ≥98%

Sigma-Aldrich

A3059

Bovine Serum Albumin

Bovine Serum Albumin heat shock fraction, protease free, fatty acid free, essentially globulin free, pH 7, ≥98%

Sigma-Aldrich

A7030

Bovine Serum Albumin

Bovine Serum Albumin heat shock fraction, lyophilized powder, essentially fatty acid free, ≥98% (agarose gel electrophoresis)

Sigma-Aldrich

A3803

Bovine Serum Albumin

technique(s)

ELISA: suitable, western blot: suitable, electrophoresis: suitable

technique(s)

ELISA: suitable, western blot: suitable

technique(s)

ELISA: suitable, blood typing: suitable, ligand binding assay: suitable

technique(s)

cell culture | mammalian: suitable

grade

for molecular biology

grade

-

grade

-

grade

-

biological source

bovine

biological source

bovine

biological source

bovine

biological source

bovine

assay

≥98%

assay

≥98%

assay

≥98%

assay

≥98% (agarose gel electrophoresis)

origin

USA origin

origin

USA origin

origin

USA origin

origin

USA origin

Quality Level

200

Quality Level

300

Quality Level

300

Quality Level

200

Application

Select the right albumin for your application with the Albumin Selection Guide.
A non-acetylated protein suitable as a blocking agent in Southern blotting. If your application requires nuclease-free BSA, we recommend our acetylated BSA for these critical applications.

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

  • Protease free
  • Suitable for blocking non-specific protein binding sites in hybridizations
  • Heat shock fractionated

Preparation Note

Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000501318
0000501318
0000484738
0000484738
0000472740

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Matthew L Fisher et al.
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Polycomb group proteins, including Ezh2, are important candidate stem cell maintenance proteins in epidermal squamous cell carcinoma. We previously showed that epidermal cancer stem cells (ECS cells) represent a minority of cells in tumors, are highly enriched in Ezh2 and
Ignacio Izeddin et al.
PloS one, 6(1), e15611-e15611 (2011-01-26)
The actin cytoskeleton of dendritic spines plays a key role in morphological aspects of synaptic plasticity. The detailed analysis of the spine structure and dynamics in live neurons, however, has been hampered by the diffraction-limited resolution of conventional fluorescence microscopy.
Marcos V A S Navarro et al.
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The bacterial second messenger bis-(3'-5') cyclic dimeric guanosine monophosphate (c-di-GMP) has emerged as a central regulator for biofilm formation. Increased cellular c-di-GMP levels lead to stable cell attachment, which in Pseudomonas fluorescens requires the transmembrane receptor LapD. LapD exhibits a
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BMC cancer, 11, 51-51 (2011-02-03)
CD26 (dipeptidyl peptidase IV, DPPIV) is a 110 kDa surface glycoprotein expressed in most normal tissues, and is a potential novel therapeutic target for selected cancers. Our work evaluates the mechanism involved in confluence-dependent CD26 expression in colon cancer. Colon
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Protocols

30-minute Immunodetection Protocol Using the SNAP i.d.® 2.0 System

The SNAP i.d. 2.0 Protein Detection System is the second generation of the SNAP i.d. method for detecting immunoreactive proteins on Western blots.

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