This 2% solution is sterilized by autoclaving and is quite stable when stored and handled properly. The product is not assigned an expiration or retest date. Products that fall into this category are subject to a warranty period of 1 year from the date of shipment. Please see the link below to review additional details available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/187/614/g6650-et-al-pis-ms.pdf
G1393
Bovine Gelatin
from bovine hide, Type B, liquid, suitable for cell culture
Gelatin solution
1 of 2 reviewers received a sample product or took part in a promotion
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Product Name
Gelatin solution, Type B, 2% in H2O, tissue culture grade, BioReagent, suitable for cell culture
biological source
bovine hide
Quality Level
sterility
sterile
type
Type B
product line
BioReagent
form
solution
packaging
pkg of 100 mL
pkg of 20 mL
concentration
2% in H2O, tissue culture grade
technique(s)
cell culture | mammalian: suitable
surface coverage
100‑200 μg/cm2
impurities
endotoxin, tested
solubility
water: soluble
Binding Specificity
Peptide Source: Fibrinogen
shipped in
ambient
storage temp.
2-8°C
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This Item | G9391 | 48722 | G2500 |
|---|---|---|---|
| sterility sterile | sterility sterile | sterility non-sterile | sterility - |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable |
| biological source bovine hide | biological source bovine skin | biological source Porcine | biological source Porcine skin |
| concentration 2% in H2O, tissue culture grade | concentration - | concentration - | concentration - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form solution | form powder | form powder | form powder |
General description
Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue. This is a hydrocolloid and is rich in glycine, proline and hydroxyproline, which imparts structural stability. It is synthesized from the alkaline digestion of collagen from bovine skin and is referred as type B. Gelatin has wide applications in food industry.[1] It takes up random coil structure after digestion from the triple helical collagen.[2] The most common source for industrial production of gelatin is slaughter byproducts. The type A gelatin and type B differ in their isoelectric pH.[3] This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objective.
Application
Gelatin solution has been used:
- for coating of cell culture plates or dishes used for embryonic stem cell culture,[4] testicular cell culture[5] and neural rosettes[6]
- in coating cell culture to improve attachment of cells
- in PCR to stabilize Taq DNA
- as a blocking reagent in western blotting
- in ELISA
- in immunochemistry
- as a component of media for species differentiation in bacteriology
- to study long-chain fatty acid-induced changes in gene expression in neonatal cardiac monocytes
- to study to test mobilization of capillary endothelium in vitro induced by effectors of angiogenesis in vivo
Biochem/physiol Actions
Gelatin has gelling property and displays surface behaviour for use in foams and adhesions.[3] It also serves as a clarification agent in fruit based juices.[1] It is a digestible protein and serves as a binding agent, thickener, stabilizer and emulsifier in food industry.[7] As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications.
Preparation Note
This product is derived lime-cured tissue bovine skin, and is a 2% solution in water.
Disclaimer
Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.
related product
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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The TGFbeta pathway is a key player for the endothelial-to-hematopoietic transition in the embryonic aorta
Lempereur A, et al.
Developmental Biology, 434(2), 292-303 (2018)
Gelatin, source, extraction and industrial applications
Mariod AA and Fadul H
Acta Scientiarum Polonorum. Technologia Alimentaria, 12(2), 135-147 (2013)
Mutations in LRRK2 impair NF-kappaB pathway in iPSC-derived neurons
de Maturana RL, et al.
Journal of Neuroinflammation, 13(1), 295-295 (2016)
Optimal culture conditions are critical for efficient expansion of human testicular somatic and germ cells in vitro
Gat I, et al.
Fertility and Sterility, 107(3), 595-605 (2017)
Prajakta Oak et al.
EMBO molecular medicine, 9(11), 1504-1520 (2017-09-20)
Neonatal chronic lung disease (nCLD) affects a significant number of neonates receiving mechanical ventilation with oxygen-rich gas (MV-O
Articles
3D 細胞培養概述。瞭解 2D 與 3D 細胞培養、3D 細胞培養的優勢，以及可用於開發 3D 細胞模型的技術。
細胞外基質 (ECM) 由細胞分泌，包圍在組織中。
癌症幹細胞培養基、球形板和癌症幹細胞標記，以培養和表徵 CSC 群。
The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.
Protocols
這份用於細胞培養的明膠塗佈方案提供了明膠類型、表面覆蓋濃度以及最佳化技巧等相關資訊。
This gelatin coating protocol for cell culture applications offers information regarding types of gelatin, surface coverage concentration, and tips for optimization.
Related Content
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For how long gelatin is stable at 2-8 °C?
1 answer-
Helpful?
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What is the molecular weight of this Gelatin?
What is the molecular weight of this Gelatin?
1 answer-
The molecular weight of this product has not been evaluated, However, the molecular weight is closely related to the Bloom Number. The estimated ranges are as follows:
Bloom Number Average Molecular Mass (Da)
50−125 (Low Bloom) 20,000−25,000
175−225 (Medium Bloom) 40,000−50,000
225−325 (High Bloom) 50,000−100,000Please see the link below to review the general datasheet for Gelatin:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/625/g9382pis.pdfHelpful?
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