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PROTIA
ProteoPrep® Immunoaffinity Albumin & IgG Depletion Kit
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About This Item
UNSPSC Code:
41106500
NACRES:
NA.56
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technique(s)
immunodepletion: suitable
storage temp.
2-8°C
1 of 4
This Item | PROTMEM | PROTDT | PROTBA |
|---|---|---|---|
| technique(s) immunodepletion: suitable | technique(s) - | technique(s) - | technique(s) - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 15-25°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
ProteoPrep® Immunoaffinity Albumin and IgG Depletion Kit might be a useful tool for novel biomarker discovery.[1]
The ProteoPrep® Immunoaffinity medium in the prepacked spin columns is a mixture of two-beaded media containing recombinantly expressed, small single-chain antibody ligands, resulting in low non-specific binding and high capacity. The ProteoPrep Immunoaffinity Albumin and IgG Depletion Kit has been designed to specifically remove albumin and IgG from human serum (25-50μL) in preparation of samples for proteomics analysis, two-dimensional electrophoresis (2DE) or liquid chromatography (LC). This kit is also effective in depleting albumin and IgG from mouse and guinea pig serum.
Application
Features and Benefits
- High Capacity removes more interfering abundant proteins
- High Specifity ensures you see an accurate and more complete proteome
- Buffer systems are compatible with 2 dimensional electrophoresis so there are no intermediate time consuming steps that might result in protein loss
Legal Information
ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class Code
10 - Combustible liquids
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Alejandro Gomez Toledo et al.
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Carmen R M Bria et al.
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Journal of cardiovascular pharmacology, 65(6), 611-619 (2015-02-26)
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My protein samples contain urea. Can I still use the ProteoPrep® Immunoaffinity Albumin & IgG Depletion Kit, Product PROTIA, for depletion?
1 answer-
The presence of urea can reduce the depletion of albumin with PROTIA. We would suggest a buffer exchange prior to depletion using either dialysis or 5 KDa NMWL cut-off spin filters.
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Can I use the ProteoPrep® Immunoaffinity Albumin & IgG Depletion Kit, Product PROTIA, on samples besides serum?
1 answer-
Besides human serum or plasma samples, we have not specifically tested other human biological fluids with this kit. However, in principle, there is no reason not to try the kit with other fluid samples.
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