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DTTP-RO

Roche

dTTP

PCR Grade, sodium salt

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Synonym(s):
Thymidine 5′-triphosphate sodium salt solution, TTP
CAS Number:
MDL number:
PubChem Substance ID:

Quality Level

Assay

99% (dNTP)

form

solution

usage

sufficient for 125,000 standard reactions (03732711001)
sufficient for 31,250 standard reactions (11969048001)
sufficient for 6,250 standard reactions (11934546001)

packaging

pkg of 1,250 μL (11969048001 [100 mM])
pkg of 4 × 1,250 μL (03732711001 [4x100 mM])
pkg of 250 μL (11934546001 [100 mM])

manufacturer/tradename

Roche

concentration

<0.9% (dNDP)
100 mM

storage temp.

−20°C

SMILES string

[Na].CC1=CN(C2CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O2)C(=O)NC1=O

InChI

1S/C10H17N2O14P3.Na.H/c1-5-3-12(10(15)11-9(5)14)8-2-6(13)7(24-8)4-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h3,6-8,13H,2,4H2,1H3,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;

InChI key

KAELIXTVSANDSE-UHFFFAOYSA-N

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This Item
11051482001DNSTP-ROT0251
dTTP PCR Grade, sodium salt

Roche

DTTP-RO

dTTP

dTTP Lithium salt

Roche

11051482001

dTTP

form

solution

form

solution

form

-

form

-

packaging

pkg of 1,250 μL (11969048001 [100 mM]), pkg of 4 × 1,250 μL (03732711001 [4x100 mM]), pkg of 250 μL (11934546001 [100 mM])

packaging

pkg of 250 μL (25 μmol)

packaging

pkg of 4 × 100 μL (11277049001 [4x10 μmol]), pkg of 40 × 100 μL (11922505001 [40x100mM])

packaging

-

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

-

concentration

<0.9% (dNDP), 100 mM

concentration

100 mM

concentration

-

concentration

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).
Thymidine 5′-triphosphate sodium salt solution is used in DNA synthesis reactions such as PCR, DNA sequencing, cDNA synthesis and molecular cloning techniques.
dTTP, PCR Grade, is a 100 mM clear, colorless solution of the sodium salt (pH 8.3).

Application

dTTP, PCR Grade, is suitable for many applications where high-quality reagents are required. Such procedures include reverse transcription (RT), polymerase chain reaction (PCR), RT-PCR, DNA labeling reactions, and sequencing/cycle sequencing analysis.

Quality

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

Physical form

solution of dTTP in water (pH 8.3).

Preparation Note

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).

Other Notes

For general laboratory use.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash


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