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  • 02065
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02065

Sigma-Aldrich

Adenosine 5′-triphosphate, immobilized on Agarose 4B

suitable for affinity chromatography, powder (lyophilized)

All Photos(1)
Synonym(s):
5′-ATP-agarose 4B
eCl@ss:
32160414
PubChem Substance ID:
24845221
NACRES:
NA.51

form

powder (lyophilized)

contains

60% lactose as stabilizer

technique(s)

affinity chromatography: suitable

capacity

≥1 μmol/mL, packed gel capacity (5′-ATP)(bound by a C6-spacer to C-8 of ATP)

storage temp.

−20°C

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Sigma-Aldrich

Sigma-Aldrich

02065

Adenosine 5′-triphosphate, immobilized on Agarose 4B

Adenosine 5′-triphosphate (ATP) assay mix lyophilized powder

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Adenosine 5′-triphosphate (ATP) assay mix

Glutathione reduced, immobilized on Agarose CL-4B suitable for affinity chromatography, powder (lyophilized)

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49739

Glutathione reduced, immobilized on Agarose CL-4B

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82492

Protein A, immobilized on Agarose CL-4B from Staphylococcus aureus

form

powder (lyophilized)

form

lyophilized powder

form

powder (lyophilized)

form

powder

contains

60% lactose as stabilizer

contains

-

contains

50% lactose as stabilizer

contains

lactose as stabilizer

technique(s)

affinity chromatography: suitable

technique(s)

-

technique(s)

affinity chromatography: suitable

technique(s)

affinity chromatography: suitable

capacity

≥1 μmol/mL, packed gel capacity (5′-ATP)(bound by a C6-spacer to C-8 of ATP)

capacity

-

capacity

-

capacity

~20 mg/mL, gel binding capacity (human IgG)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

General description

The lactose-stabilizer must be removed prior to use by washing the gel onto filter with water or buffer.

Application

Adenosine 5′-triphosphate, immobilized on Agarose 4B (5′-ATP-agarose 4B) is intended for use in affinity chromatography. 5′-ATP-agarose 4B has been shown to bind enzymes with affinity to 5′-ATP. 5′-ATP-agarose 4B may be considered for used with enzymes that bind to other ATP-agarose and sepharose affinity media or beads.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Purification of cofactor-dependent enzymes by affinity chromatography.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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