This product has an endotoxin specification of ≤ 1 EU/ml or 0.1 ng/mL. The lot specific values are reported in the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/d6429#product-documentation
D6429
DMEM - high glucose
With sodium bicarbonate, ʟ-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM
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Product Name
Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
HEPES: no
NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
L-glutamine: yes
glucose: high
shipped in
ambient
storage temp.
2-8°C
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This Item | D6546 | D5796 | D1145 |
---|---|---|---|
sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
form liquid | form liquid | form liquid | form liquid |
components HEPES: no | components NaHCO3: yes | components NaHCO3: yes | components L-glutamine: no |
storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
General description
Application
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Hi, What is the concentration of endotoxin detected in DMEM high glucose media D6429?
1 answer-
Helpful?
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What is the concentration of Sodium Bicarbonate and Sodium Pyruvate in it?
1 answer-
This formulation contains 3.7 g/L sodium bicarbonate and 110 mg/L sodium pyruvate. The composition of this product is published on page 3 of the product information sheet. Please see the link below to access this document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/318/256/d6429for.pdf
Helpful?
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After opening a bottle of medium: how long can I use this bottle ?(stored at 4C°)
1 answer-
Provided that the product is handled and stored properly and has not been contaminated, the medium will be stable up to the expiration date. This date is provided on the label and the lot-specific Certificate of Analysis.
Helpful?
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I would like to inquire about the amino acid formulation in DMEM (#6429, #6546). Specifically, could you provide information on the concentration of aspartate and glutamate in these formulations? Thank you very much.
1 answer-
The composition of this product is published on page 3 of the product information sheet. Please see the link below to access this document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/318/256/d6429for.pdf
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