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MilliporeSigma

D6429

Sigma-Aldrich

DMEM - high glucose

With sodium bicarbonate, ʟ-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

Dulbecco’s Modified Eagle’s Medium - high glucose
2 of 6 reviewers took part in a promotion

Synonym(s):

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

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500 ML
$36.30
1 L
$58.70
6 X 500 ML
$190.00
6 X 1 L
$317.00
24 X 500 ML
$682.00

$36.30


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500 ML
$36.30
1 L
$58.70
6 X 500 ML
$190.00
6 X 1 L
$317.00
24 X 500 ML
$682.00

About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

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$36.30


In StockDetails


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Product Name

Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

HEPES: no
NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
L-glutamine: yes
glucose: high

shipped in

ambient

storage temp.

2-8°C

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Show Differences

1 of 4

This Item
D6546D5796D1145
sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

liquid

form

liquid

form

liquid

form

liquid

components

HEPES: no
NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
L-glutamine: yes
glucose: high

components

NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
HEPES: no
L-glutamine: no
glucose: high

components

NaHCO3: yes
L-glutamine: yes
glucose: high
phenol red: yes
sodium pyruvate: no
HEPES: no

components

L-glutamine: no
sodium pyruvate: no
glucose: high
phenol red: no
NaHCO3: yes
HEPES: no

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".
Dulbecco′s Modified Eagle′s Medium - high glucose has been used for cell culture.[1][2][3]

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Certificates of Analysis (COA)

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Questions

1–4 of 4 Questions  
  1. Hi, What is the concentration of endotoxin detected in DMEM high glucose media D6429?

    1 answer
    1. This product has an endotoxin specification of ≤ 1 EU/ml or 0.1 ng/mL. The lot specific values are reported in the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/d6429#product-documentation

      Helpful?

  2. What is the concentration of Sodium Bicarbonate and Sodium Pyruvate in it?

    1 answer
    1. This formulation contains 3.7 g/L sodium bicarbonate and 110 mg/L sodium pyruvate. The composition of this product is published on page 3 of the product information sheet. Please see the link below to access this document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/318/256/d6429for.pdf

      Helpful?

  3. After opening a bottle of medium: how long can I use this bottle ?(stored at 4C°)

    1 answer
    1. Provided that the product is handled and stored properly and has not been contaminated, the medium will be stable up to the expiration date. This date is provided on the label and the lot-specific Certificate of Analysis.

      Helpful?

  4. I would like to inquire about the amino acid formulation in DMEM (#6429, #6546). Specifically, could you provide information on the concentration of aspartate and glutamate in these formulations? Thank you very much.

    1 answer
    1. The composition of this product is published on page 3 of the product information sheet. Please see the link below to access this document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/318/256/d6429for.pdf

      Helpful?

Reviews

2 of 6 reviewers took part in a promotion

Active Filters

  1. Atlanta, GA
    • Reviews 4
    • Votes 9
    5 out of 5 stars.

    D6171 with HEPES modification

    The best media to culture HEK293FT cells. The HEPES modification seems beneficial during calcium-phosphate transfection by perhaps stabilizing pH.

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  2. 5 Ratings-Only Reviews

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