D4635
2′-Deoxycytidine 5′-triphosphate disodium salt
≥95%
Sinónimos:
dCTP-Na2
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| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 10 mg | Comprobar disponibilidad del carrito | $105.00 | |
| 25 mg | Comprobar disponibilidad del carrito | $244.00 | |
| 100 mg | Comprobar disponibilidad del carrito | $685.00 | |
| 1 g | Comprobar disponibilidad del carrito | $3,270.00 |
Acerca de este artículo
Fórmula empírica (notación de Hill):
C9H14N3Na2O13P3
Número CAS:
Peso molecular:
511.12
NACRES:
NA.51
PubChem Substance ID:
UNSPSC Code:
41106305
MDL number:
Assay:
≥95%
Form:
powder
Storage temp.:
−20°C
$105.00
Comprobar disponibilidad del carrito
Servicio técnico
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Permítanos ayudarleQuality Level
assay
≥95%
form
powder
shipped in
dry ice
storage temp.
−20°C
SMILES string
[Na+].[Na+].NC1=NC(=O)N(C=C1)[C@H]2C[C@H](O)[C@@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)O2
InChI
1S/C9H16N3O13P3.2Na/c10-7-1-2-12(9(14)11-7)8-3-5(13)6(23-8)4-22-27(18,19)25-28(20,21)24-26(15,16)17;;/h1-2,5-6,8,13H,3-4H2,(H,18,19)(H,20,21)(H2,10,11,14)(H2,15,16,17);;/q;2*+1/p-2/t5-,6+,8+;;/m0../s1
InChI key
ABWVCNMFYVEBIB-CDNBRZBRSA-L
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General description
2′-Deoxycytidine 5′-triphosphate (dCTP) is synthesized either by de novo pathway or from the multiple phosphorylation steps of cytidine. It is deaminated to deoxyuridine monophosphate (dUMP) which is convertible to deoxythymidine triphosphate (dTTP).
Application
2′-Deoxycytidine 5′-triphosphate disodium salt has been used:
- in polymerase chain reaction mix for the amplification of Alternaria (AM) toxin DNA[1]
- to spike oligonucleotide sample for capillary electrophoresis (CE) analysis[2]
- as a component in adenosine 5′-monophosphate (AMP) luciferase assay[3]
2′-Deoxycytidine 5′-triphosphate disodium salt is one of the nucleoside triphosphates used for DNA polymerase driven reactions such as polymerase chain reaction, DNA sequencing and other molecular biology methods.
Biochem/physiol Actions
2′-Deoxycytidine 5′-triphosphate (dCTP) is used to synthesize DNA by DNA polymerases or reverse transcriptases. dCTP is an allosteric regulator of deoxycytidylate (dCMP) deaminase.[4] It is used in studies of nucleotide pools, nucleotide selectivity and enzyme specificity studies, such as the preferred incorporation of dCTP by Rev1, a γ-family DNA polymerase.[5]
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Genetics: Analysis of Genes and Genomes (2009)
Xiaoli Xiang et al.
Analytical biochemistry, 460, 22-28 (2014-05-27)
A luciferase-based method was developed for measurement of 5'-adenylylsulfate (APS) reductase (APR), an enzyme of the reductive sulfate assimilation pathway in prokaryotes and plants. APR catalyzes the two-electron reduction of APS and forms sulfite and adenosine 5'-monophospahate (AMP). The luciferase-based
Dynamics of pyrimidine deoxynucleoside triphosphate pools in relationship to DNA synthesis in 3T6 mouse fibroblasts
Nicander B and Reichard P
Proceedings of the National Academy of Sciences, 80(5), 1347-1351 (1983)
Paola Ferraro et al.
Nucleic acids research, 38(6), e85-e85 (2009-12-17)
Eukaryotic cells contain a delicate balance of minute amounts of the four deoxyribonucleoside triphosphates (dNTPs), sufficient only for a few minutes of DNA replication. Both a deficiency and a surplus of a single dNTP may result in increased mutation rates
Jessica A Brown et al.
Biochemistry, 49(26), 5504-5510 (2010-06-04)
Rev1, a Y-family DNA polymerase, contributes to spontaneous and DNA damage-induced mutagenic events. In this paper, we have employed pre-steady-state kinetic methodology to establish a kinetic basis for nucleotide selection by human Rev1, a unique nucleotidyl transferase that uses a
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| D4635-1G | 04061826690567 |
| D4635-25MG | 04061833006887 |
| D4635-100MG | 04061833565575 |
| D4635-10MG | 04061833006870 |
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