Please see the link below to review the Product Information sheet which lists the complete formulation, including amino acids and the associated concentrations:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/171/436/d6421for.pdf
D6421
DMEM/F-12
With HEPES, sodium bicarbonate and sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
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Sinónimos:
DME/Nutrient Mixture F-12 Ham, DMEM Hams F12, DME/F-12, 1:1 mixture
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
500 mL | Fecha estimada de envío21 de septiembre de 2026desdeMILWAUKEE | $55.80 |
6 x 500 mL | Comprobar disponibilidad del carrito | $291.00 |
24 x 500 mL | Comprobar disponibilidad del carrito | $1,050.00 |
Acerca de este artículo
$55.80
Ahorre hasta 22%
Fecha estimada de envío21 de septiembre de 2026Detalles
Servicio técnico
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Permítanos ayudarleNombre del producto
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With 15 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Quality Segment
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
sodium pyruvate: 0.055 g/L
L-glutamine: no
phenol red: yes
NaHCO3: yes
HEPES: 15 mM
glucose: 3.15 g/L
shipped in
ambient
storage temp.
2-8°C
General description
Listen to an Audio Overview
A measured mixture of chemically defined ingredients makes up a basal medium, which is a type of complex media. DMEM is a medium supplemented with serum and sometimes with tryptose phosphate broth (TPB). DMEM/F-12 is a completely defined synthetic medium, which is protein-free.
A measured mixture of chemically defined ingredients makes up a basal medium, which is a type of complex media. DMEM is a medium supplemented with serum and sometimes with tryptose phosphate broth (TPB). DMEM/F-12 is a completely defined synthetic medium, which is protein-free.
Application
Suitable for growth of wide range of cell types in low serum conditions.
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is used in cell culture & proliferation assays. It is also used in bone marrow-derived macrophages culture.
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is used in cell culture & proliferation assays. It is also used in bone marrow-derived macrophages culture.
DMEM:F12 is a 50:50 mixture of DMEM and Ham′s F12 media that has proven to be useful in a wide range of cell culture applications, especially when supplemented with fetal bovine serum (FBS). Ham′s Nutrient Mixture F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L cells. It is frequently used with dialyzed serum, hormones, selenium, and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat, and has been proven to be an excellent cloning medium for myeloma and hybridoma cells.
Preparation Note
Supplement with 0.365 g/L L-glutamine.
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Este artículo | |||
|---|---|---|---|
| description With 15 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture | description With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture | description With L-glutamine and 15 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture | description liquid, suitable for cell culture |
| sterility sterile-filtered | sterility sterile-filtered | sterility - | sterility sterile |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form liquid | form liquid | form powder | form liquid |
| components sodium pyruvate: 0.055 g/L | components glucose: 3.15 g/L | components sodium pyruvate: 0.055 g/L | components sodium pyruvate: 0.055 g/L |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
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Clase de almacenamiento
12 - Non Combustible Liquids
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Is it possible to get a complete list of media components, such as the amino acids and their concentrations please. I could not find it in the specification sheet.
1 answer-
Helpful?
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May I confirm if it is acceptable to use 0.1 M HCl and 0.1 M NaOH to balance the pH of the medium (D6421)? Are there any specific recommendations or precautions for this adjustment process to maintain the integrity and performance of the medium?
1 answer-
The pH of DMEM F-12 can be adjusted to 6.9 - 7.1 using NaOH 1N or HCl 1N.
The pH of bicarbonate buffered solutions usually rises 0.1 - 0.2 units during filtration
It is recommended to add HCl or NaOH drop-wise while continuously stirring to avoid drastic pH swings. Use the smallest volume possible to avoid significantly altering the osmolarity of the medium. Maintain sterile conditions when adjusting pH for cell culture use.Helpful?
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