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NACRES:
NA.52
UNSPSC Code:
41105501
Technique(s):
RNA purification: suitable
Usage:
sufficient for 50 purifications
usage
sufficient for 50 purifications
Quality Segment
sustainability
Sustainability features
technique(s)
RNA purification: suitable
General description
Norgen Biotek′s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including viral RNA, from a wide range of plant and filamentous fungal species. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The procedure is rapid and convenient, as it does not rely on the use of liquid nitrogen in order to homogenize the samples.
Application
For the rapid purification of total RNA (including microRNA) from plants and fungi.
Purified RNA is of the highest quality and can be used in a number of downstream applications, including:
- qRT-PCR
- Reverse transcription PCR
- Northern blotting
- RNAse protection
- Primer extension
- Expression array assays
- Next Generation Sequencing
Features and Benefits
- Isolate RNA from a wide range of plant and filamentous fungi samples.
- Robust lysis buffer is suitable for challenging samples such as pine needle or grape leaf.
- Isolate a diversity of RNA species from large mRNA, microRNA, and viral RNA.
- Purification of high yield and quality RNA.
- No phenol/chloroform extraction required.
- Liquid nitrogen is not required for homogenization
Other Notes
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
This product aligns with one or more of the 12 Principles of Green ChemistryThis product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Palabra de advertencia
Warning
Códigos de peligro
Indicaciones de precaución
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2
Clase de almacenamiento
10 - Combustible liquids
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