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F7425

ANTI-FLAG® antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Sinónimos:

Anti-ddddk, Anti-dykddddk

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Talla/SKUDisponibilidadPrecio
0.2 mg

Envío estimado HOYdesdeMILWAUKEE

$655.00

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UNSPSC Code:
12352203
NACRES:
NA.32
MDL number:
MFCD02262912
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
DB, FLAG purification, IF, IP, WB CL
Citations:
-

$655.00

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biological source

rabbit

Quality Segment

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

all

concentration

~0.8 mg/mL

technique(s)

FLAG purification: suitable, dot blot: 1-2.5 μg/mL, immunoprecipitation (IP): 4-8 μg using amino terminal FLAG-BAP fusion protein from E. coli crude lysate, indirect immunofluorescence: 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK, western blot (chemiluminescent): 1-2.5 μg/mL using an E. coli periplasmic extract expressing an N-terminal FLAG fusion protein

isotype

IgG

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

General description

El anticuerpo policlonal de afinidad anti-FLAG de conejo ANTI-FLAG reconoce el epítopo FLAG situado en las proteínas de fusión FLAG. Este anticuerpo reacciona con los extremos N-terminal, Met-N-terminal, C-terminal de las proteínas de fusión FLAG.
El epítopo FLAG es una proteína de ocho aminoácidos con un sitio de escisión por enterocinasa.

Immunogen

FLAG; secuencia peptídica DYKDDDDK

Application

El anticuerpo reconoce el epítopo FLAG situado en las proteínas de fusión etiquetadas con FLAG en los extremos N-terminal o C-terminal mediante la aplicación de ensayos de inmunotransferencia por puntos (dot blot), inmunotransferencia, inmunoprecipitación e inmunocitoquímica.
Conozca más detalles del producto en nuestro portal de la aplicación FLAG®.

Biochem/physiol Actions

El epítopo FLAG desempeña un papel crucial en la purificación mediante inmunoafinidad de las proteínas de fusión.

Physical form

Disolución en disolución salina tamponada con fosfato 0,01 M, pH 7,4, que contiene seroalbúmina bovina al 1 % y acida sódica 15 mM.

Preparation Note

Purificado mediante cromatografía de afinidad en una columna portadora del péptido inmunizante.

Other Notes

Ganador del premio CiteAb 2024 como proveedor de éxito en la investigación del Parkinson

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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1 of 1

Este artículo
F2555F1804F9291
ANTI-FLAG® antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

Millipore

F7425

ANTI-FLAG® antibody produced in rabbit

Rabbit Monoclonal ANTI-FLAG® Clone SIGI-25 clone SIG1-25, ascites fluid

Sigma-Aldrich

F2555

Rabbit Monoclonal ANTI-FLAG® Clone SIGI-25

ANTI-FLAG® M2 monoclonal antibody produced in mouse 1.0-1.2 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

Sigma-Aldrich

F1804

ANTI-FLAG® M2 monoclonal antibody produced in mouse

ANTI-FLAG® M2 monoclonal antibody produced in mouse clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Sigma-Aldrich

F9291

ANTI-FLAG® M2 monoclonal antibody produced in mouse

description

affinity isolated antibody, buffered aqueous solution

description

clone SIG1-25, ascites fluid

description

1.0-1.2 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

description

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

biotin conjugate

antibody form

affinity isolated antibody

antibody form

ascites fluid

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

biological source

rabbit

biological source

rabbit

biological source

mouse

biological source

mouse

clone

polyclonal

clone

SIG1-25, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

species reactivity

all

species reactivity

-

species reactivity

all

species reactivity

all

technique(s)

western blot (chemiluminescent): 1-2.5 μg/mL using an E. coli periplasmic extract expressing an N-terminal FLAG fusion protein, indirect immunofluorescence: 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK, FLAG purification: suitable, dot blot: 1-2.5 μg/mL, immunoprecipitation (IP): 4-8 μg using amino terminal FLAG-BAP fusion protein from E. coli crude lysate

technique(s)

immunocytochemistry: 1:125-1:250 using transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein fixed with paraformaldehyde/Triton™ X-100, western blot: 1:250-1:500 using extracts of transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein, indirect ELISA: suitable, FLAG purification: suitable

technique(s)

FLAG purification: suitable

technique(s)

-

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Clase de almacenamiento

12 - Non Combustible Liquids

Punto de inflamación (°F)

Not applicable

Punto de inflamación (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number

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Questions

1–9 of 9 Questions  

  1. Can this AB (F7425) used for mouse 30um thick section. mouse will be perfused with 4% PFA and than cryopreserved. thanks

    1 answer

    1. This product has been recommended for use in immunofluorescence and indirect immunofluorescence at 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK. It has not been tested for use in IHC applications. The end-user will need to optimize the assay. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/207/353/f7425dat-ms.pdf

      In general, antibodies work well on 5-15 uM sections, for 30 uM sections, blocking /permeabilization steps and antibody dilution, the incubation time will need to be optimized by end user. Please see the links below to review an alternative anti-Flag antibody that may be more suitable for this application. Note that this product is a mouse monoclonal antibody:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/175/747/f1804bul-ms.pdf
      https://www.sigmaaldrich.com/product/sigma/f1804

      Helpful?

  2. Is there a recommended isotype control for this antibody?

    1 answer

    1. Any rabbit IgG such as product I5006 would be an appropriate isotype control for this antibody.

      Helpful?

  3. Is Product F7425, ANTI-FLAG® from rabbit, a monoclonal or polyclonal?

    1 answer

    1. F7425 is a polyclonal antibody from rabbit.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. How much Product F7425, ANTI-FLAG® from rabbit, should be used in western blotting and ELISA applications?

    1 answer

    1. For a Western blot from a PAGE run (preferably) under denaturing conditions, a 1:400 dilution of this antibody should be used.  A 1-10 μg/ml concentration should be used when using thie antibody in an ELISA application.

      Helpful?

  6. What are the positive controls that can be used with Product F7425, ANTI-FLAG® from rabbit?

    1 answer

    1. The positive controls that can be used are as follows:Amino-terminal FLAG-BAP fusion protein, Product No. P7582Carboxy-terminal FLAG-BAP fusion protein, Product No. P7457Amino-terminal Met-FLAG-BAP fusion protein, Product No. P5975.

      Helpful?

  7. Is there any references for the use of Product F7465, ANTI-FLAG® from rabbit, in immunohistochemistry or western blotting?

    1 answer

    1. K. Schafer and T. Braun, Monoclonal Anti-FLAG Antibodies React with a New Isoform of Rat Mg++ Dependent Protein Phosphatase. Biochemical and Biophysical Research Communications, 207, 708.

      Helpful?

  8. What is the concentration of Product F7425, ANTI-FLAG® from rabbit?

    1 answer

    1. The concentration is 0.9 mg protein per ml.

      Helpful?

  9. How to get rid of background when using Product F7465, ANTI-FLAG® from rabbit,?

    1 answer

    1. To reduce non-specific background on tissue staining, a small amount of Triton X-100 (Product No. X100 or T8787), can be added to the wash buffers and antibody incubation buffers. Try a Triton X-100 concentration of 0.1% to start. Tween 20 (Product No. P5927) can also be used.

      Helpful?

Reviews

Active Filters

  1. Nottingham
    • Review 1
    • Votes 0
    5 out of 5 stars.

    Western blotting

    I HAVE USED THIS ANTIBODY ALOT DURING MY RESEARCH. AND IT HAS WORKED PERFECTLY.

    Helpful?