- Dereplication and chemotaxonomical studies of marine algae of the Ochrophyta and Rhodophyta phyla.
Dereplication and chemotaxonomical studies of marine algae of the Ochrophyta and Rhodophyta phyla.
Marine drugs (2015-05-06)
Robert Brkljača, Emrehan Semih Gӧker, Sylvia Urban
PMID25942092
RESUMEN
Dereplication and chemotaxonomic studies of six marine algae of the Ochrophyta and one of the Rhodophyta phyla resulted in the detection of 22 separate compounds. All 16 secondary metabolites, including four new compounds (16-19), could be rapidly dereplicated using HPLC-NMR and HPLC-MS methodologies in conjunction with the MarinLit database. This study highlights the advantages of using NMR data (acquired via HPLC-NMR) for database searching and for the overall dereplication of natural products.
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Óxido de deuterio, 99.9 atom % D, contains 0.05 wt. % 3-(trimethylsilyl)propionic-2,2,3,3-d4 acid, sodium salt
Sigma-Aldrich
Agua, for embryo transfer, sterile-filtered, BioXtra, suitable for mouse embryo cell culture
Sigma-Aldrich
Óxido de deuterio, 99.9 atom % D, contains 0.75 wt. % 3-(trimethylsilyl)propionic-2,2,3,3-d4 acid, sodium salt
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Acetonitrilo solution, contains 0.1 % (v/v) trifluoroacetic acid, suitable for HPLC
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Diclorometano, suitable for HPLC, ≥99.9%, contains 40-150 ppm amylene as stabilizer