Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Please refer to Enzyme Commission number: 3.1.1.3 which can be found on the product landing page:
https://www.sigmaaldrich.com/product/sigma/l1754
L1754
Lipase from Candida rugosa
Type VII, ≥700 unit/mg solid
Synonym(s):
Triacylglycerol acylhydrolase, Triacylglycerol lipase
Sign In to View Organizational & Contract Pricing.
Select a Size
Pricing and availability is not currently available.
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-619-9
MDL number:
Skip To
Product Name
Lipase from Candida rugosa, Type VII, ≥700 unit/mg solid
InChI key
QWZUIMCIEOCSJF-CHHCPSLASA-N
InChI
1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
biological source
(from Candida cylindracea Type VII)
type
Type VII
form
lyophilized
specific activity
≥700 unit/mg solid
storage condition
(Tightly closed. Dry)
technique(s)
cell based assay: suitable
color
beige
white
solubility
water: slightly soluble
storage temp.
2-8°C
Quality Level
Looking for similar products? Visit Product Comparison Guide
1 of 4
This Item | 62316 | 62302 | 62285 |
|---|---|---|---|
| specific activity ≥700 unit/mg solid | specific activity ≥2 U/mg | specific activity 15-25 U/mg | specific activity ~50 U/mg |
| biological source fungus (candida rugosa) | biological source fungus (Candida rugosa) | biological source fungus (Candida rugosa) | biological source - |
| technique(s) cell based assay: suitable | technique(s) analytical sample preparation: suitable | technique(s) - | technique(s) - |
| form lyophilized | form powder | form lyophilized solid, powder (fine) | form powder |
| solubility water: slightly soluble | solubility water: slightly soluble | solubility - | solubility - |
| storage condition (Tightly closed. Dry) | storage condition dry at room temperature | storage condition - | storage condition - |
Application
Lipase from Candida rugosa has been used:
- to synthesize dextran fatty acid esters[1]
- to study the effect of lipase on conjugated linoleic acid (CLA1 and CLA2) production in the presence of sunflower oil and castor oil[2]
- to prepare pH-imprinted enzyme for lipase-catalyzed transesterification of dextran T-40 with vinyl decanoate[3]
Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.
Biochem/physiol Actions
Lipases are enzymes and biocatalysts that catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids. They are useful for biotransformation reactions.[4] It possesses broad substrate tolerance[4] and is highly stereo- and regioselective.[1] Lipases mediate lipid transport. Elevated levels of serum lipase are observed in pancreatitis[5]. Lipases are the preferred catalysts for long-chain fatty acids.[1] Lipases can be used in a wide range of solvents, in both aqueous and non-aqueous media, and thus have found use in applications like organic synthesis.
Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).
Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).
Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
General description
Research area: Cell Structure
Lipases are found in pancreatic secretions. This class of enzymes contains α and β hydrolase folds.[5] Candida rugosa produces multiple lipase isoenzymes that have 80% sequence homology.[6]
Lipases are found in pancreatic secretions. This class of enzymes contains α and β hydrolase folds.[5] Candida rugosa produces multiple lipase isoenzymes that have 80% sequence homology.[6]
This product contains lactose as a carrier.
Other Notes
One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.2 at 37 °C. (This is equivalent to approx. 10 microliters of CO2 in 30 minutes.)
related product
Product No.
Description
Pricing
used together
Product No.
Description
Pricing
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Ashok Kumar et al.
Biological procedures online, 18, 2-2 (2016-01-15)
Lipases are industrial biocatalysts, which are involved in several novel reactions, occurring in aqueous medium as well as non-aqueous medium. Furthermore, they are well-known for their remarkable ability to carry out a wide variety of chemo-, regio- and enantio-selective transformations.
Taresh P Khobragade et al.
Frontiers in bioengineering and biotechnology, 9, 757062-757062 (2021-10-26)
Herein, we report the development of a multi-enzyme cascade using transaminase (TA), esterase, aldehyde reductase (AHR), and formate dehydrogenase (FDH), using benzylamine as an amino donor to synthesize the industrially important compound sitagliptin intermediate. A panel of 16 TAs was
Marco Filice et al.
Nature protocols, 7(10), 1783-1796 (2012-09-08)
This protocol describes the regioselective deprotection of single hydroxyls in peracetylated monosaccharides and disaccharides by enzymatic or chemoenzymatic strategies. The introduction of a one-pot enzymatic step by using immobilized biocatalysts obviates the requirement to carry out tedious workups and time-consuming
Enzymatic synthesis of oligo- and polysaccharide fatty acid esters.
van den Broek, et al.
Carbohydrate Polymers, 93, 65-72 (2013)
Lipase
Pirahanchi Y and SharmaS
Biochemistry (2019)
Related Content
-
Why is the definition of Enzymatic activity "One Unit will Hydrolyze 1 µequivalent of Fatty Acid from TG” instead of “One Unit will liberate 1 µequivalent of Fatty Acid from TG”. In fact, a Fatty acid is not hydrolyzed but liberated from TG hydrolysis.
1 answer-
Helpful?
-
-
which polysaccharide was utilized in the lyophilized form of CRL as stabilizing agent, and how to remove it before working with this formula to avoid the impact of polysaccharides on immobilization process.
1 answer-
This product contains lactose as a carrier. Information on how to remove the lactose has not been determined.
Helpful?
-
-
What is the stability of Product L1754, Lipase from Candida rugosa solutions, and how should they be stored?
1 answer-
Solutions in 50% glycerol may be stored at -20°C up to six months. Avoid repeated freeze/thaw cycles. It is best to store aliquots in working volumes.
Helpful?
-
-
What is the solubility of Product L1754, Lipase from Candida rugosa?
1 answer-
This product is soluble in water at 1 mg/mL.
Helpful?
-
-
What is the difference between Candida rugosa and Candida cylindracea?
1 answer-
These organisms are identical strains. C. rugosa was assigned to what was previously named C. cylindracea by the ATCC.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service