This product may be reconstituted at 50 - 200 ug/mL in sterile 4 mM HCl containing at least 0.1% BSA or HSA. Stock solutions may be stored refrigerated at 2 - 8°C for up to 1 month or at -70°C, in aliquots, for up to 3 months. Avoid repeated freeze/that cycles.
L1754
Lipase from Candida rugosa
Type VII, ≥700 unit/mg solid
Synonym(s):
Triacylglycerol acylhydrolase, Triacylglycerol lipase
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| Size/SKU | Availability | Price |
|---|---|---|
5 g | Available to ship TODAYfromMILWAUKEE | $68.00 |
10 g | Available to ship TODAYfromMILWAUKEE | $93.30 |
25 g | Available to ship TODAYfromMILWAUKEE | $163.00 |
100 g | Available to ship TODAYfromMILWAUKEE | $490.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-619-9
EC Number:
MDL number:
Specific activity:
≥700 unit/mg solid
Biological source:
(from Candida rugosa)
Quality Segment
biological source
(from Candida rugosa)
type
Type VII
form
lyophilized
specific activity
≥700 unit/mg solid
storage condition
(Tightly closed. Dry)
technique(s)
cell based assay: suitable
color
beige, white
solubility
water: slightly soluble
storage temp.
2-8°C
InChI
1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
InChI key
QWZUIMCIEOCSJF-CHHCPSLASA-N
General description
Research area: Cell Structure
Lipases are found in pancreatic secretions. This class of enzymes contains α and β hydrolase folds. Candida rugosa produces multiple lipase isoenzymes that have 80% sequence homology.
Lipases are found in pancreatic secretions. This class of enzymes contains α and β hydrolase folds. Candida rugosa produces multiple lipase isoenzymes that have 80% sequence homology.
This product contains lactose as a carrier.
Application
Lipase from Candida rugosa has been used:
- to synthesize dextran fatty acid esters
- to study the effect of lipase on conjugated linoleic acid (CLA1 and CLA2) production in the presence of sunflower oil and castor oil
- to prepare pH-imprinted enzyme for lipase-catalyzed transesterification of dextran T-40 with vinyl decanoate
Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.
Biochem/physiol Actions
Lipases are enzymes and biocatalysts that catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids. They are useful for biotransformation reactions. It possesses broad substrate tolerance and is highly stereo- and regioselective. Lipases mediate lipid transport. Elevated levels of serum lipase are observed in pancreatitis. Lipases are the preferred catalysts for long-chain fatty acids. Lipases can be used in a wide range of solvents, in both aqueous and non-aqueous media, and thus have found use in applications like organic synthesis.
Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).
Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).
Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Other Notes
One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.2 at 37 °C. (This is equivalent to approx. 10 microliters of CO2 in 30 minutes.)
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This Item | |||
|---|---|---|---|
| description Type VII, ≥700 unit/mg solid | description lyophilized, powder (fine), 15-25 U/mg | description lyophilized powder, ≥4,000 units/mg solid (using olive oil) | description powder, slightly brown, ~1 U/mg |
| biological source (from Candida rugosa) | biological source fungus (Candida rugosa) | biological source - | biological source - |
| specific activity ≥700 unit/mg solid | specific activity 15-25 U/mg | specific activity ≥4,000 units/mg solid (using olive oil) | specific activity ~1 U/mg |
| technique(s) cell based assay: suitable | technique(s) - | technique(s) - | technique(s) - |
| form lyophilized | form powder (fine), lyophilized solid | form lyophilized powder | form powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| solubility water: slightly soluble | solubility - | solubility - | solubility - |
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Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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Lipase from Candida rugosa（L1754）は、製品情報に「水に1 mg/mLまで可溶」と記載されています。しかし、10 mM PBS緩衝液および25 mM Tris緩衝液では十分に溶解せず、不溶物が残りました。より高濃度に溶解させるための推奨条件はありますか。例えば、推奨される緩衝液、添加剤、pH、塩濃度などがありましたらご教示ください。酵素活性を維持したまま溶解度を向上させる方法があれば知りたいです。
1 answer-
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Why is the definition of Enzymatic activity "One Unit will Hydrolyze 1 µequivalent of Fatty Acid from TG” instead of “One Unit will liberate 1 µequivalent of Fatty Acid from TG”. In fact, a Fatty acid is not hydrolyzed but liberated from TG hydrolysis.
1 answer-
Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Please refer to Enzyme Commission number: 3.1.1.3 which can be found on the product landing page:
https://www.sigmaaldrich.com/product/sigma/l1754Helpful?
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which polysaccharide was utilized in the lyophilized form of CRL as stabilizing agent, and how to remove it before working with this formula to avoid the impact of polysaccharides on immobilization process.
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This product contains lactose as a carrier. Information on how to remove the lactose has not been determined.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the difference between Candida rugosa and Candida cylindracea?
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These organisms are identical strains. C. rugosa was assigned to what was previously named C. cylindracea by the ATCC.
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What is the solubility of Product L1754, Lipase from Candida rugosa?
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This product is soluble in water at 1 mg/mL.
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What is the stability of Product L1754, Lipase from Candida rugosa solutions, and how should they be stored?
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Solutions in 50% glycerol may be stored at -20°C up to six months. Avoid repeated freeze/thaw cycles. It is best to store aliquots in working volumes.
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