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IPVH00010

Immobilon® -P PVDF Membrane

1 roll, 27 cm x 3.75 m, 0.45 µm pore size, transfer membrane

Synonym(s):

Western blotting membrane, blotting membrane, transfer membrane

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Size/SKUAvailabilityPrice
1 ea
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$821.00

About This Item

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric, radioactive
Pore size:
0.45 μm pore size
Compatibility:
for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination

$821.00

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Product Name

Immobilon®-P PVDF Membrane, 1 roll, 27 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

Quality Segment

material

PVDF membrane, plain filter, white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable, electrophoresis transfer: suitable, western blot: suitable

filter L × W

27 cm × 3.75 m

pore size

0.45 μm pore size

capacity

160 μg/cm2 adsorption capacity (insulin), 215 μg/cm2 adsorption capacity (BSA), 294 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination

detection method

chemiluminescent, colorimetric, fluorometric, radioactive

shipped in

ambient

General description

Immobilon-P transfer membrane is recommended for most Western blotting applications, especially proteins greater than 20 kDa. Immobilon-P membrane has excellent protein retention, high physical strength, and broad chemical compatibility, making it ideal for a variety of staining applications and reprobing in immunodetection methods.

Features and Benefits

Immobilon-P PVDF Membrane:
  • Won′t crack, curl or fracture when cut
  • Low background
  • Superior staining capabilities
  • Can be reprobed multiple times

Other Notes

Filter Code: IPVH
Primary Binding Mechanisms: Electrostatic, hydrophobic
Replaces: INCP00010

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

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IPVH10100IPVH08100IPVH08130
Immobilon®-P PVDF Membrane 1 roll, 27 cm x 3.75 m, 0.45 &#181;m pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

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Immobilon®-P PVDF Membrane 10 sheets, 8 cm x 10 cm, 0.45 &#181;m pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

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IPVH08100

Immobilon®-P PVDF Membrane

Immobilon®-P PVDF Membrane 10 sheets, 8.5 cm x 13.5 cm, 0.45 &#181;m pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

Millipore

IPVH08130

Immobilon®-P PVDF Membrane

description

1 roll, 27 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

description

10 sheets, 10 cm x 10 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

description

10 sheets, 8 cm x 10 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

description

10 sheets, 8.5 cm x 13.5 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

400

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

material

plain filter, white filter, PVDF membrane

material

plain filter, white filter, PVDF membrane

material

plain filter, PVDF membrane, white filter

material

plain filter, PVDF membrane, white filter

detection method

fluorometric, chemiluminescent, colorimetric, radioactive

detection method

fluorometric, chemiluminescent, colorimetric, radioactive

detection method

radioactive, fluorometric, colorimetric, chemiluminescent

detection method

colorimetric, fluorometric, radioactive, chemiluminescent

compatibility

for use with Amido black, for use with Toluidine blue, for use with Transillumination, for use with Colloidal gold, for use with CPTS, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with India ink, for use with Ponceau-S red, for use with Coomassie brilliant blue

compatibility

for use with India ink, for use with Ponceau-S red, for use with Coomassie brilliant blue, for use with Transillumination, for use with Colloidal gold, for use with CPTS, for use with Toluidine blue, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Amido black

compatibility

for use with Amido black, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with India ink, for use with Coomassie brilliant blue, for use with Ponceau-S red, for use with Transillumination, for use with CPTS, for use with Toluidine blue, for use with Colloidal gold

compatibility

for use with Ponceau-S red, for use with Amido black, for use with India ink, for use with Transillumination, for use with Toluidine blue, for use with CPTS, for use with Coomassie brilliant blue, for use with Colloidal gold, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby

capacity

215 μg/cm2 adsorption capacity (BSA), 160 μg/cm2 adsorption capacity (insulin), 294 μg/cm2 adsorption capacity (goat IgG)

capacity

215 μg/cm2 adsorption capacity (BSA), 294 μg/cm2 adsorption capacity (goat IgG), 160 μg/cm2 adsorption capacity (insulin)

capacity

215 μg/cm2 adsorption capacity (BSA), 294 μg/cm2 adsorption capacity (goat IgG), 160 μg/cm2 adsorption capacity (insulin)

capacity

215 μg/cm2 adsorption capacity (BSA), 294 μg/cm2 adsorption capacity (goat IgG), 160 μg/cm2 adsorption capacity (insulin)

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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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Questions

  1. What is the recommended blocking agent for PVDF membranes in a western blot, particularly when using Casein? Is casein suitable for PVDF membranes considering its suitability as a blocking agent?

    1 answer

    1. The recommended blocking agent for PVDF membranes in a Western blot, including when using Casein, as per ther Immobilon®P Transfer Membrane user guide is using a blocking buffer called Block-CH buffer (Cat. No. WBAVDCH01) or a blocking agent such as bovine serum albumin, casein, or nonfat dry milk in wash buffer at a concentration of 0.5-5% (w/v).

      Regarding the suitability of Casein as a blocking agent for PVDF membranes, in-house testing has been conducted with IPVH00010 PVDF membrane, and Casein has been found to be suitable. However, it is important to note that users often choose the blocking agent that works best for their specific needs. Therefore, if one prefers to use Casein as a blocking agent with PVDF membrane (IPVH00010), it can be employed as long as it does not interfere with the samples and antibodies being used.

      Helpful?

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