SCC162
CFTE29o- Human Cystic Fibrosis Tracheal Epithelial Cell Line
Human
Synonym(s):
CFTE29o, CFTE-29o-
Select a Size
| Size/SKU | Availability | Price |
|---|---|---|
1 vial | Check Cart for Availability | $1,870.00 |
About This Item
$1,870.00
Product Name
CFTE29o- Human Cystic Fibrosis Tracheal Epithelial Cell Line, CFTE29o- human cystic fibrosis tracheal epithelial cell line is a useful model for cystic fibrosis research and for studying chloride ion transport and ion transport in human airways.
biological source
human
technique(s)
cell based assay: suitable, cell culture | mammalian: suitable
shipped in
ambient
storage temp.
-140 to -196°C
General description
CFTE29o- is a human CF tracheal epithelial cell line, derived from a 21-yr old male CF patient homozygous for the ΔF508 CFTR mutation and immortalized with the origin-of-replication defective SV40 plasmid (pSVori-) . The cell line express keratin, indicating an epithelial cell origin, along with a calcium-dependent cell adhesion protein, cellCAM 120/80 , and ZO-1 indicating the ability to form tight junctions. CFTE29o- displays ion transport properties characteristic of cystic fibrosis such as defective cAMP-dependent chloride transport. Upon treatment with the calcium ionophore, ionomycin, CFTE29o- secretes chloride, albeit at reduced levels relative to normal cells. The cells express CFTR mRNA, and small amounts of CFTR protein as assessed by Western blot hybridization . Karyotype analysis indicates that 70% of the cells contain two copies of chromosome 7 .
CFTE29o- is a useful model for cystic fibrosis and for studying chloride ion transport and ion transport in human airways. The cells are useful for testing candidate chemotherapeutic drugs for their potential as treatments for ameliorating CF pathologies.
Application
Biochem/physiol Actions
Preparation Note
Analysis Note
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, C, and HIV-1 & 2 viruses as assessed by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
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| description CFTE29o- human cystic fibrosis tracheal epithelial cell line is a useful model for cystic fibrosis research and for studying chloride ion transport and ion transport in human airways. | description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA. | description lyophilized powder (from clotted whole blood) | description from human(normal adult), multiple organ |
| technique(s) cell culture | mammalian: suitable, cell based assay: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture: suitable using | mammalian | technique(s) - |
| biological source human | biological source human esophagus | biological source human | biological source human (normal adult) |
| storage temp. -140 to -196°C | storage temp. - | storage temp. 2-8°C | storage temp. - |
| shipped in ambient | shipped in - | shipped in - | shipped in - |
Storage Class
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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