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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Check Cart for Availability | $504.00 |
About This Item
NACRES:
NA.21
UNSPSC Code:
12352204
$504.00
Check Cart for Availability
form
tablet
manufacturer/tradename
Roche
packaging
pkg of Foil blister pack
solubility
aqueous buffer: soluble
storage temp.
room temp
General description
The cOmplete™ Lysis-M mammalian cell protein extraction reagent contains a mild ready-to-use detergent (in 25mM bicine buffer, pH 7.6) that efficiently lyses mammalian cells and eliminates the need for scraping, sonication, or freeze-thaw cycles. It is required for the gentle extraction of proteins from both the cytoplasm and the nucleus of cultured mammalian cells. Protein yields obtained with this kit are 20 to 25% higher compared to three cycles of freeze-thaw, and approximately 20% higher than 2 minutes of sonication (with 50% pulse). Extracted proteins can be further purified or analyzed in downstream applications. Efficient lysis of mammalian cells occurs in only 5 minutes at room temperature.
The ready-to-use cOmplete™ Mini Protease Inhibitor Cocktail Tablets, included in the kit, function optimally with the kit′s Lysis-M reagent. The cOmplete™ Mini Protease Inhibitor Tablets allow the inhibition of a broad spectrum of serine, cysteine, and metalloproteases, as well as calpains. cOmplete™ Lysis-M reagent helps in protein extraction and simultaneously inhibit protease activity during the initial extraction steps. cOmplete™ Mini Protease Inhibitor Tablets contain both irreversible and reversible protease inhibitors.
The ready-to-use cOmplete™ Mini Protease Inhibitor Cocktail Tablets, included in the kit, function optimally with the kit′s Lysis-M reagent. The cOmplete™ Mini Protease Inhibitor Tablets allow the inhibition of a broad spectrum of serine, cysteine, and metalloproteases, as well as calpains. cOmplete™ Lysis-M reagent helps in protein extraction and simultaneously inhibit protease activity during the initial extraction steps. cOmplete™ Mini Protease Inhibitor Tablets contain both irreversible and reversible protease inhibitors.
Application
Lysis-M reagent is compatible with many different applications:
Note: cOmplete™ Mini tablets (included in the kit) contain EDTA (ethylenediaminetetraacetic acid). If the protein of interest is to be purified by IMAC (immobilized metal-chelate affinity chromatography), e.g., Poly-His tagged recombinant proteins, EDTA has to be removed (e.g., by dialysis) prior to the chromatography. Alternatively, the cOmplete™ Lysis-M EDTA-free product can be used.
- reporter assays (e.g., β-galactosidase, luciferase, chloramphenicol acetyltransferase)
- immunoassays (e.g., Western blots, ELISAs, RIAs)
- protein assays (e.g., protein kinase A, protein kinase C, and tyrosine kinase)
- protein purification
Note: cOmplete™ Mini tablets (included in the kit) contain EDTA (ethylenediaminetetraacetic acid). If the protein of interest is to be purified by IMAC (immobilized metal-chelate affinity chromatography), e.g., Poly-His tagged recombinant proteins, EDTA has to be removed (e.g., by dialysis) prior to the chromatography. Alternatively, the cOmplete™ Lysis-M EDTA-free product can be used.
Features and Benefits
- Preserves protein structure, since reagents will not denature or otherwise alter proteins.
- Saves money, since one product protects against many proteases.
- Saves steps, since extracted proteins may be used directly in many downstream procedures.
Packaging
1 kit containing 2 components. Each tablet is sufficient for 10 mL of extract. For the lysis of up to 100 g of mammalian cells.
Preparation Note
Intended use: Simultaneous extraction and protection of soluble proteins from mammalian cells.
Sample material: Mammalian cells in culture (e.g., Cos-7 cells, harvested at confluency).
Time required: 5 minutes (cell lysis at room temperature).
Number of extractions: 20 (10 ml each).
Effective against: Serine proteases, cysteine proteases, metalloproteases.
Note: Each cOmplete, Mini tablet is concentrated enough to inhibit proteases in 10 ml of extract.
Sample material: Mammalian cells in culture (e.g., Cos-7 cells, harvested at confluency).
Time required: 5 minutes (cell lysis at room temperature).
Number of extractions: 20 (10 ml each).
Effective against: Serine proteases, cysteine proteases, metalloproteases.
Note: Each cOmplete, Mini tablet is concentrated enough to inhibit proteases in 10 ml of extract.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
cOmplete is a trademark of Roche
1 of 1
This Item | |||
|---|---|---|---|
| description Individually packed in foil blister packs | description Individually packed in foil blister packs | description Tablets supplied in foil blister packs. | description Tablets provided in EASYpacks |
| form tablet | form tablet | form tablet | form tablet |
| solubility aqueous buffer: soluble | solubility aqueous buffer: soluble | solubility water: soluble | solubility aqueous buffer: soluble, water: soluble |
| storage temp. room temp | storage temp. room temp | storage temp. 2-8°C | storage temp. 2-8°C |
| manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename Roche |
| packaging pkg of Foil blister pack | packaging pkg of Foil blister pack | packaging pkg of 30 tablets (individually packed in foil blister packs) | packaging pkg of 30 tablets (individually packed in foil blister packs) |
Kit Components Only
Product No.
Description
- Lysis-M Reagent for protein extraction from mammalian cells
- cOmplete Mini Protease Inhibitor Cocktail Tablets supplied in EASYpacks
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
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