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Bovine Serum Albumin

Name: Bovine Serum Albumin
Brand: SIAL
Price: 263 USD
Availability: InStock

lyophilized powder, essentially IgG free, ≥96% (agarose gel electrophoresis)

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Synonym(s):

Albumin bovine serum, BSA, Bovine albumin

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Size/SKU	Availability	Price
25 g	Check Cart for Availability	$263.00

About This Item

CAS Number:

9048-46-8

UNSPSC Code:

12352202

EC Number:

232-936-2

NACRES:

NA.25

MDL number:

MFCD00130384

eCl@ss:

32160409

Biological source:

bovine

Assay:

≥96% (agarose gel electrophoresis)

Form:

lyophilized powder

Technique(s):

flow cytometry: suitable, immunofluorescence: suitable

Impurities:

IgG free, ≤25 ng/mg protein IgG

Concentration:

<25 ng/mg IgG

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Properties

biological source

bovine

Quality Segment

200

assay

≥96% (agarose gel electrophoresis)

form

lyophilized powder

purified by

heat shock fractionation

packaging

poly bottle of

origin

USA origin

concentration

<25 ng/mg IgG

technique(s)

flow cytometry: suitable, immunofluorescence: suitable

impurities

IgG free, ≤25 ng/mg protein IgG

pH

solubility

water: soluble (40 mg/ml)

UniProt accession no.

P02769

foreign activity

Blue tongue virus, none detected, Vesicular Statitis virus, none detected

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

Description

General description

Bovine serum albumin (BSA) is a 66 kDa protein consisting of three domains with two subdomains under each. It is a α-helical, globular and non-glycosylated protein. BSA belongs to the serum albumins family. It has 17-disulfide bonds.

Application

Bovine Serum Albumin has been used in:

the washing of the rabbit tissue sections in immunofluorescence microscopy
incubation of red blood cells (RBC) for flow cytometry analysis
as a component of Hanks′ balanced salt solution with Ca²⁺ and Mg²⁺ (HBSS++) for eosin-5-maleimide staining and RBC washing in magnetic circular dichroism measurements

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Bovine Serum Albumin (BSA) is a transporter for drugs, hormones and fatty acids. It is used as a blocking agent in enzyme linked immunosorbent assay (ELISA) for preventing non-specific binding of antigens and antibodies to the microtiter plates. It is a crucial component of the cell culture media and favors embryonic stem cells (hESC) differentiation. BSA shares structural features with human serum albumin. Its high solubility, low cost and purity and interaction with surfactant is exploited in cosmetic and pharmaceutical industry.

Features and Benefits

Essentially IgG free (immunoglobulin-free)
Heat shock fractionated

Preparation Note

Prepared using heat shock fractionation.

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

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This Item	A9543	A2058	A9056
Sigma-Aldrich A9085 Bovine Serum Albumin	$Bovine Serum Albumin heat shock fraction, low endotoxin, pH 7, ≥98%$ Sigma-Aldrich A9543 Bovine Serum Albumin	Sigma-Aldrich A2058 Bovine Serum Albumin	$Bovine Serum Albumin heat shock fraction, microbiologically tested, ≥96% (agarose gel electrophoresis)$ Sigma-Aldrich A9056 Bovine Serum Albumin
description lyophilized powder, essentially IgG free, ≥96% (agarose gel electrophoresis)	description heat shock fraction, low endotoxin, pH 7, ≥98%	description lyophilized powder, essentially IgG-free, low endotoxin, BioReagent, suitable for cell culture	description heat shock fraction, microbiologically tested, ≥96% (agarose gel electrophoresis)
technique(s) immunofluorescence: suitable, flow cytometry: suitable	technique(s) ELISA: suitable, electrophoresis: suitable, western blot: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
assay ≥96% (agarose gel electrophoresis)	assay ≥98%	assay ≥95% (agarose gel electrophoresis)	assay ≥96% (agarose gel electrophoresis)
biological source bovine	biological source bovine	biological source bovine	biological source bovine
origin USA origin	origin USA origin	origin New Zealand origin	origin USA origin
Quality Level 200	Quality Level 200	Quality Level 300	Quality Level 200
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder

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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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