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S5016

Sucrose

ACS reagent

Synonym(s):

α-D-Glc-(1→2)-β-D-Fru, α-D-Glucopyranosyl β-D-fructofuranoside, β-D-Fructofuranosyl-α-D-glucopyranoside, D(+)-Saccharose, Sugar

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About This Item

Linear Formula:
C12H22O11
CAS Number:
Molecular Weight:
342.30
UNSPSC Code:
12352201
NACRES:
NA.21
PubChem Substance ID:
EC Number:
200-334-9
Beilstein/REAXYS Number:
90825
MDL number:
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biological source

sugar cane

Quality Segment

grade

ACS reagent

form

powder or crystals

optical activity

[α]25/D +66.3 to +66.8°(lit.)

technique(s)

HPLC: suitable, cryopreservation: suitable, immunohistochemistry: suitable

impurities

≤0.0008 meq/g Titratable acid, ≤0.005% Insoluble matter, ≤0.05% Invert sugar

ign. residue

≤0.01%

loss

≤0.03% loss on drying, 105°C

color

white

useful pH range

5.5-7.5 (25 °C, 342 g/L)

mp

185-187 °C (lit.)

solubility

soluble 342 g/L at 20 °C (completely)

anion traces

chloride (Cl-): ≤0.005%, sulfate, sulfite (as SO42-): ≤0.005%

cation traces

Fe: ≤5 ppm, heavy metals: ≤5 ppm (by ICP-OES)

application(s)

agriculture
cell analysis
life science and biopharma
metabolomics

storage temp.

room temp

SMILES string

OC[C@H]1O[C@H](O[C@]2(CO)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C12H22O11/c13-1-4-6(16)8(18)9(19)11(21-4)23-12(3-15)10(20)7(17)5(2-14)22-12/h4-11,13-20H,1-3H2/t4-,5-,6-,7-,8+,9-,10+,11-,12+/m1/s1

InChI key

CZMRCDWAGMRECN-UGDNZRGBSA-N

General description

Sucrose, a disaccharide consisting of glucose and fructose, serves as an energy source in numerous biological processes. In cell culture, sucrose functions as a cryoprotectant, preserving cell viability during freezing and thawing while also helping to maintain osmotic balance and enhance cell survival. Moreover, sucrose finds utility as a supplement in plant, insect, and bacterial culture media, and it is applied in various enzymatic assays. In biomedical research, it plays a role as an osmolyte, an algal metabolite, a Saccharomyces cerevisiae metabolite, an E. coli metabolite, and a mouse metabolite.

Application

Sucrose has been used
  • in a growth medium to study the molecular and biochemical aspects of plant-pathogen interactions and plant immune responses
  • as a cryoprotectant in a study involving antibiotic-induced changes to the gut microbiota and their effects on neuroimmune interactions

Biochem/physiol Actions

Sucrose serves as a vital energy source, undergoing metabolic conversion to produce adenosine triphosphate (ATP) for cellular functions and cellular respiration. In plant biology, sucrose is central to photosynthesis and acts as a storage carbohydrate in various plant parts. In Cell Biology, it helps maintain osmotic balance in cells, acts as a cryoprotectant in laboratory settings, and participates in biosynthesis, leading to the formation of secondary metabolites.

Features and Benefits

  • Suitable for cell biology and biochemical research
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Tested for anion and cation traces
  • ACS grade sucrose, sourced from sugar cane

Other Notes

For additional information on our range of Biochemicals, please complete this form.
To gain a comprehensive understanding of our extensive range of Disaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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This Item
S1888S5391SX1075
description

ACS reagent

description

≥99.5% (GC), BioReagent, suitable for cell culture, suitable for insect cell culture

description

≥99.5% (GC), Grade II, suitable for plant cell culture

description

Meets ACS Specifications GR ACS

technique(s)

HPLC: suitable, cryopreservation: suitable, immunohistochemistry: suitable

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | plant: suitable

technique(s)

-

biological source

sugar cane

biological source

sugar cane

biological source

sugar cane

biological source

-

grade

ACS reagent

grade

-

grade

-

grade

-

Quality Level

300

Quality Level

300

Quality Level

300

Quality Level

-

solubility

soluble 342 g/L at 20 °C (completely)

solubility

H2O: 500 mg/mL

solubility

H2O: 0.5 g/mL, clear, colorless to faintly yellow

solubility

-

form

powder or crystals

form

crystals

form

powder

form

crystals


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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Questions

1–4 of 4 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. After dilution to approximately 50% w/v and subsequent autoclaving, what is the optimal storage temperature? Should the solution be stored at 4°C (refrigerator) or at room temperature?

    1 answer
    1. Sterile solutions of this product may be stored at room temperature. For such a concentrated solution in a refrigerator or even in a cold laboratory, one might see that some of the sucrose crystallizes out over time; it can be redissolved by warming the solution.

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  4. Is this NIST traceable?

    1 answer
    1. This product is not NIST traceable. Currently, a NIST traceable standard is not available.

      Helpful?

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