Key Documents

Anti-Human IgM (μ-chain specific)−Peroxidase antibody produced in goat

Name: Anti-Human IgM (μ-chain specific)−Peroxidase antibody produced in goat
Brand: SIGMA
Price: 295 USD
Availability: InStock

affinity isolated antibody

Synonym(s):

Goat Anti-Human IgM (μ-chain specific)−HRP

Select a Size

Change View

Size/SKU	Availability	Price
1 mL	Available to ship TODAYfromMILWAUKEE	$295.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162464

Conjugate:

peroxidase conjugate

Clone:

polyclonal

Application:

DB, ELISA (d), IHC (p)

Citations:

$295.00

Available to ship TODAYDetails

Request a Custom Order

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

goat

Quality Segment

300

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

human

technique(s)

direct ELISA: 1:50,000, dot blot: 1:100,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

B cells predominantly secrete IgM antibodies as pentamers, but may also secrete them as hexamers which are capable of activating the complement cascade more efficiently.
Goat anti-human IgM (mu-chain specific)-peroxidase antibody is specific for human IgM and does not bind to other human Igs. Furthermore, the antibody does not react with mouse and rat IgG. Hence, the antibody conjugate yields reduced background with mouse or rat samples.

Immunogen

human IgM

Application

Anti- Human IgM was used in an ELISA assay to determine the concentration of IgM secreted by CH12 B lymphoma cells stimulated by LPS.

Goat anti-human IgM (mu-chain specific)-peroxidase antibody can be used:

for dot blot (1:100,000) and immunohistochemistry (1:200 using formalin-fixed, paraffin-embedded sections) applications.
in immunoblotting
sandwich immunoassay

Biochem/physiol Actions

IgM plays a key role in engulfing of the apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and higher risk for infections. IgM displays polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating clearance of tissue based molecules.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	A6907	A0295	A6029
Sigma-Aldrich A0420 Anti-Human IgM (μ-chain specific)−Peroxidase antibody produced in goat	Sigma-Aldrich A6907 Anti-Human IgM (μ-chain specific)−Peroxidase antibody produced in goat	Sigma-Aldrich A0295 Anti-Human IgA (α-chain specific)−Peroxidase antibody produced in goat	Sigma-Aldrich A6029 Anti-Human IgG (γ-chain specific)−Peroxidase antibody produced in goat
description affinity isolated antibody	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody	description affinity isolated antibody, buffered aqueous solution
species reactivity human	species reactivity human	species reactivity human	species reactivity human
biological source goat	biological source goat	biological source goat	biological source goat
conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate peroxidase conjugate
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
technique(s) dot blot: 1:100,000 (chemiluminescent), direct ELISA: 1:50,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200	technique(s) direct ELISA: 1:10,000	technique(s) direct ELISA: 1:50,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, western blot: suitable, dot blot: 1:80,000	technique(s) direct ELISA: 1:10,000
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice