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Anti-Monkey IgG (whole molecule)−Peroxidase antibody produced in rabbit

Name: Anti-Monkey IgG (whole molecule)−Peroxidase antibody produced in rabbit
Brand: SIGMA
Price: 274 USD
Availability: InStock

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Rabbit Anti-Monkey IgG (whole molecule)-HRP

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Size/SKU	Availability	Price
1 mL	Available to ship TODAYfromMILWAUKEE	$274.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162609

Conjugate:

peroxidase conjugate

Clone:

polyclonal

Application:

DB, ELISA (d)

Citations:

$274.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

monkey

technique(s)

direct ELISA: 1:45,000, dot blot: 1:160,000 (direct chemiluminescence assay)

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Immunoglobulins (Igs) have two heavy (H) and two light (L) chains, held together by disulphide linkages. They belong to the immunoglobulin super-family. The heavy chain has one variable N-terminal region and three to four constant (CH1-CH4) C-terminal regions. The light chain has one variable and constant region. IgGs are subclassified into IgG1, IgG2, IgG3 and IgG4.

Monkey IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in monkey serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Horseradish Peroxidase (HRP) is an enzyme that catalyzes the conversion of chromogenic substrates such as o-phenylenediamine (OPD), 4-chloro-1-naphthol 3,3′,5,5′-tetramethylbenzidine (TMB), 3,3′-Diaminobenzidine (DAB) or 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS); chemiluminescent substrates such as CPS-3 (enhanced luminal) and fluorogenic substrates such as Ampliflu^™ Red into detectable chromophores, light-emitters or fluorescers, respectively.

Rabbit polyclonal anti-Monkey IgG (whole molecule)−Peroxidase antibody recognizes monkey IgG in vitro and in monkey serum and biological fluids.

Immunogen

monkey IgG (rhesus)

Application

Rabbit polyclonal anti-Monkey IgG (whole molecule)−Peroxidase antibody may be used to detect and quantitate the level of IgG in monkey serum and biological fluids via chromogenic, chemoluminescent or fluorogenic immunochemical or immunohistochemical techniques. It may also be used as a secondary antibody in assays that use monkey IgG as the primary antibody. It may be use to detect parasite-specific antibodies in monkey sera, ELISA assays were performed using HRP-conjugated rabbit anti-monkey IgG as the secondary.

Anti-Monkey IgG (whole molecule)−Peroxidase antibody produced in rabbit has been used in ELISA of serum samples.

Biochem/physiol Actions

Digestion of IgG by papain results in generation of fragment antigen binding (Fab). Pepsin digestion of IgG results in fragment crystallizable (Fc). Fc regions of IgG antibody have enormous therapeutic potential and are exploited for the development of therapeutic antibodies. IgG1 class is the most abundant and its deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions and IgG4 is associated with asymptomatic infection. The IgG levels in monkey show age related increase. In monkeys, IgG subtypes comprises the predominant antibodies during immune response after hepatitis B surface antigen (HBsAg) vaccination. The human and monkey IgG sequences show divergence only in the hinge region and hence exploited for therapeutic antibody testing.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Legal Information

Ampliflu is a trademark of Sigma-Aldrich Co. LLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A8917	A6917	A9042
Sigma-Aldrich A2054 Anti-Monkey IgG (whole molecule)−Peroxidase antibody produced in rabbit	$Anti-Bovine IgG (whole molecule)−Peroxidase antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich A8917 Anti-Bovine IgG (whole molecule)−Peroxidase antibody produced in rabbit	Sigma-Aldrich A6917 Anti-Horse IgG (whole molecule)−Peroxidase antibody produced in rabbit	$Anti-Dog IgG (whole molecule)−Peroxidase antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich A9042 Anti-Dog IgG (whole molecule)−Peroxidase antibody produced in rabbit
description affinity isolated antibody, buffered aqueous solution	description IgG fraction of antiserum, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description IgG fraction of antiserum, buffered aqueous solution
species reactivity monkey	species reactivity bovine	species reactivity -	species reactivity -
conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate peroxidase conjugate
technique(s) direct ELISA: 1:45,000, dot blot: 1:160,000 (direct chemiluminescence assay)	technique(s) dot blot: 1:80,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000, direct ELISA: 1:20,000	technique(s) direct ELISA: 1:10,000	technique(s) direct ELISA: 1:25,000, dot blot: 1:40,000 using direct chemiluminescence assay using 10 ng dog IgG/dot. Luminol plus enhancer was used as substrate.
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
antibody form affinity isolated antibody	antibody form IgG fraction of antiserum	antibody form affinity isolated antibody	antibody form IgG fraction of antiserum
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal

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Pictograms

GHS07

Signal Word

Warning

Hazard Codes

H317

Precautionary Statements

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

WGK

WGK 2

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