A2306
Monoclonal Anti-Rabbit Immunoglobulins−Alkaline Phosphatase antibody produced in mouse
clone RG-16, purified immunoglobulin, buffered aqueous glycerol solution
Synonym(s):
Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)
Select a Size
| Size/SKU | Availability | Price |
|---|---|---|
0.2 mL | Check Cart for Availability | $268.00 |
About This Item
biological source
mouse
Quality Segment
conjugate
alkaline phosphatase conjugate
antibody form
purified immunoglobulin
antibody product type
secondary antibodies
clone
RG-16, monoclonal
form
buffered aqueous glycerol solution
species reactivity
rabbit
should not react with
human, pig, chicken, bovine, horse, guinea pig, turkey, rat, canine, goat, feline, sheep
technique(s)
direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160 using human tonsil and Cat. No. I8635 as the primary antibody, western blot: 1:160,000-1:320,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg per well)
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Application
- indirect enzyme linked immunosorbent assay (ELISA)
- immunoblotting
- immunohistochemistry
Biochem/physiol Actions
Mouse monoclonal anti-rabbit immunoglobulins-alkaline phosphatase antibody binds to an epitope on the heavy chain of rabbit IgG, IgA, and IgM. The product stains bands at the intact whole molecule and at the heavy chains in an immunoblot of denatured, non-reduced rabbit immunoglobulins. Reduction of rabbit immunoglobulins destroys the epitope. In ELISA, the product shows no cross reaction with human serum or tissue preparations, nor with human IgA, IgG, or IgM.
Physical form
Disclaimer
1 of 1
This Item | |||
|---|---|---|---|
| description clone RG-16, purified immunoglobulin, buffered aqueous glycerol solution | description clone RG-16, purified immunoglobulin, lyophilized powder | description affinity isolated antibody, buffered aqueous solution | description clone RG-16, purified immunoglobulin, buffered aqueous solution |
| conjugate alkaline phosphatase conjugate | conjugate peroxidase conjugate | conjugate alkaline phosphatase conjugate | conjugate biotin conjugate |
| technique(s) western blot: 1:160,000-1:320,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg per well), direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160 using human tonsil and Cat. No. I8635 as the primary antibody | technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissue, direct ELISA: 1:30,000, western blot (chemiluminescent): 1:160,000 | technique(s) western blot: 1:50 dilution, direct ELISA: 1:3,000 using IgG, IgA, IgM | technique(s) direct ELISA: 1:60,000, western blot: 1:200,000-1:400,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,500 |
| biological source mouse | biological source mouse | biological source goat | biological source mouse |
| antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form affinity isolated antibody | antibody form purified immunoglobulin |
| species reactivity rabbit | species reactivity rabbit | species reactivity mouse | species reactivity rabbit |
| isotype IgG1 | isotype IgG1 | isotype - | isotype IgG1 |
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Storage Class
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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