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A5545

Anti-Guinea Pig IgG (whole molecule)−Peroxidase antibody produced in rabbit

Name: Anti-Guinea Pig IgG (whole molecule)−Peroxidase antibody produced in rabbit
Brand: SIGMA
Price: 207 EUR

affinity isolated antibody, buffered aqueous solution

Sinonimo/i:

Rabbit Anti-Guinea pig IgG (whole molecule)−HRP

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A voi/SKU	Disponibilità	Prezzo
1 mL	Per informazioni sulla disponibilità, contatta il Servizio Clienti.	207,00 €

Informazioni su questo articolo

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162368

Conjugate:

peroxidase conjugate

Clone:

polyclonal

Application:

DB, ELISA (d), IHC (p)

Citations:

207,00 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.

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Proprietà

biological source

rabbit

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

guinea pig

technique(s)

direct ELISA: 1:50,000-1:80,000, dot blot: 1:50,000-1:100,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400-1:800

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Descrizione

General description

An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. It has two subclasses of IgG- IgG1 and IgG2.

Immunogen

guinea pig IgG

Application

Anti-Guinea Pig IgG (whole molecule) Peroxidase antibody produced in rabbit has been used in diphtheria serology enzyme-linked immunosorbent assay (ELISA) and immunoblotting.

Biochem/physiol Actions

IgGs are glycoprotein antibodies that modulate several immune responses. Guinea pig IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-guinea pig IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.

Immunoglobulin G (IgG) is the major class and most abundant immunoglobulin of immune system released by plasma cells. IgG mediate immune response with respect to the type of antigen and is found in blood or extracellular fluid. IgG defends against bacterial and viral infections. Maternal IgG crosses from mother to fetus through the placental barrier by pinocytosis to provide passive immunity for the neonate and protect against infections.

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. IgG1 helps in complement fixation through alternate pathway and IgG2 fixes complement by conventional mechanism. It mainly helps in immune defense. Guinea Pig is a highly preferred laboratory animal to study the local and anaphylactic reactions.

Rabbit anti-guinea pig IgG (whole molecule)-peroxidase antibody binds to guinea pig IgG.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Store at -20 °C for long term use. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots at -20 °C. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Questo articolo	A6792	A2054	A5670
Sigma-Aldrich A5545 Anti-Guinea Pig IgG (whole molecule)−Peroxidase antibody produced in rabbit	Sigma-Aldrich A6792 Anti-Dog IgG (whole molecule)−Peroxidase antibody produced in rabbit	Sigma-Aldrich A2054 Anti-Monkey IgG (whole molecule)−Peroxidase antibody produced in rabbit	Sigma-Aldrich A5670 Anti-Pig IgG (whole molecule)−Peroxidase antibody produced in rabbit
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
technique(s) direct ELISA: 1:50,000-1:80,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400-1:800, dot blot: 1:50,000-1:100,000 (chemiluminescent)	technique(s) direct ELISA: 1:10,000	technique(s) direct ELISA: 1:45,000, dot blot: 1:160,000 (direct chemiluminescence assay)	technique(s) direct ELISA: 1:40,000, dot blot: 1:150,000 (chemiluminescent)
species reactivity guinea pig	species reactivity -	species reactivity monkey	species reactivity pig
conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate peroxidase conjugate
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice

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pictograms

GHS07

signalword

Warning

hcodes

H317

pcodes

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Classe di stoccaggio

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c