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Anti-Rat IgG (whole molecule)−Peroxidase antibody produced in rabbit

Name: Anti-Rat IgG (whole molecule)−Peroxidase antibody produced in rabbit
Brand: SIGMA
Price: 421 USD
Availability: InStock

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Rabbit Anti-Rat IgG (whole molecule)−HRP

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Pack Size	SKU	Availability	Price
2 mL		Available to ship TODAYfromMILWAUKEE	$421.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162813

Conjugate:

peroxidase conjugate

Clone:

polyclonal

Application:

ELISA (d), IHC (p), WB CL

Citations:

$421.00

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Properties

biological source

rabbit

Quality Level

200

conjugate

peroxidase conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

rat

technique(s)

direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500, western blot (chemiluminescent): 1:5,000 using detecting rat primary antibody

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

IgG (Immunoglobulin G) antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Immunoglobulins have two heavy chains and two light chains connected by disulfide bond. It is a glycoprotein which mainly helps in immune defense. IgG is a major class of immunoglobulin. Rat has five immunoglobulin classes- IgM, IgG, IgA, IgD and IgGE. IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c.

Immunogen

purified rat IgG

Application

Anti-Rat IgG (whole molecule) Peroxidase antibody produced in rabbit has been used in western blotting[1] and enzyme linked immunosorbent assay (ELISA).

Biochem/physiol Actions

Anti-Rat IgG (whole molecule) antibody recognizes rat (immunoglobulin G) IgG and is determined by immunoelectrophoresis (IEP) versus normal rat serum and rat IgG prior to conjugation.

IgGs are glycoprotein antibodies that modulate several immune responses. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A5795	A6066	A8792
$Anti-Rat IgG (whole molecule)−Peroxidase antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich A9542 Anti-Rat IgG (whole molecule)−Peroxidase antibody produced in rabbit	Sigma-Aldrich A5795 Anti-Rat IgG (whole molecule)−Peroxidase antibody produced in rabbit	Sigma-Aldrich A6066 Anti-Rat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit	$Anti-Human IgG (whole molecule)−Peroxidase antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich A8792 Anti-Human IgG (whole molecule)−Peroxidase antibody produced in rabbit
species reactivity rat	species reactivity rat	species reactivity -	species reactivity human
technique(s) direct ELISA: 1:40,000, western blot (chemiluminescent): 1:5,000 using detecting rat primary antibody, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500	technique(s) direct ELISA: 1:50,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:750, western blot (chemiluminescent): 1:80,000-1:160,000 using rat PC12 cell extract	technique(s) direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100, western blot: 1:30,000-1:60,000	technique(s) direct ELISA: 1:40,000, dot blot: 1:80,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150
conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate alkaline phosphatase conjugate	conjugate peroxidase conjugate
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
antibody form IgG fraction of antiserum	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form IgG fraction of antiserum
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal

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pictograms

GHS08

signalword

Danger

hcodes

H317,H334

pcodes

P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Global Trade Item Number

SKU	GTIN
A9542-2ML	04061833402917

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