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B20221

Collagenase-DNase I blend

Name: Collagenase-DNase I blend
Brand: SIGMA

Tissue dissociation blend, lyophilized powder, suitable for cell culture

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あなたへ/カタログ番号	在庫状況	単価

この商品について

NACRES:

NA.54

UNSPSC Code:

12352204

Specific activity:

(Collagenase (FALGPA assay) 1.0-5.0 units/ml in 10 ml HBSS (#H6648)), (Deoxyribonuclease I activity: 200-2000 Kunitz units/ml in 10 ml HBSS (#H6648))

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特性

Quality Segment

200

form

lyophilized powder

specific activity

(Collagenase (FALGPA assay) 1.0-5.0 units/ml in 10 ml HBSS (#H6648)), (Deoxyribonuclease I activity: 200-2000 Kunitz units/ml in 10 ml HBSS (#H6648))

storage temp.

-10 to -25°C

詳細

General description

The collagenase-DNase I blend is a tissue dissociation enzyme cocktail for cell isolation. The tissue dissociation process consists in the release of functional and viable cells from animal tissue, with minimal impact on the cell’s viability. The main reagent used for the tissue dissociation process is Collagenase. Collagenases are enzymes that break down the native collagen that holds animal tissues together. The second enzyme found in our blend is Deoxyribonuclease I (DNase I). DNase I, is an endonuclease of the DNase family, with a specific role for the degradation of DNA. DNase I is one of the most well characterized endonucleases of mammalian origin. During tissue dissociation, parts of the cells are lysed resulting in a release of DNA. Monomolecular DNA may cause clumping of cells, so addition of DNase I to our blend leads to a degradation of this extracellular DNA, thereby avoiding the loss of cells from undesired clumping.

Biochem/physiol Actions

Collagenase: Effective release of cells from tissue requires the action of collagenase enzymes. Collagenase is activated by four-gram atom calcium (Ca2+) per mole enzyme. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Collagenase treatment can cause some cells to die. Typically, concentrations varying from 0.1 to 5 mg/mL are used for digestion. The duration of reaction varies from 15 minutes to several hours and yields satisfactory cell dissociation without causing too much cell death. DNase: DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity.

Preparation Note

For a working concentration of: Collagenase of 1 mg/ml and Deoxyribonuclease I of 0.1 mg/ml, reconstitute the lyophilized powder with 10 ml of Hank’s Balanced Salt solution with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate (#H6648)

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当該品目	B20222	SCR139	SCR140
Sigma-Aldrich B20221 Collagenase-DNase I blend	Sigma-Aldrich B20222 Collagenase - Hyaluronidase blend	Sigma-Aldrich SCR139 Animal Free Collagenase/Dispase Blend I	Sigma-Aldrich SCR140 Animal Free Collagenase/Dispase Blend II
description Tissue dissociation blend, lyophilized powder, suitable for cell culture	description Tissue dissociation blend, lyophilized powder, suitable for cell culture	description Animal Free Collagenase/Dispase Blend I is a mixture of animal free collagenase, type B, and neutral protease/dispase, and is inteneded to be used when a more potent enzymatic dissociatation solution than collagnaease is desired.	description Animal Free Collagenase/Dispase Blend II is a mixture of animal free collagenase, type B, and neutral protease/dispase, and is inteneded to be used when a more potent enzymatic dissociatation solution than collagnaease is desired.
specific activity (Collagenase (FALGPA assay) 1.0-5.0 units/ml in 10 ml HBSS (#H6648)), (Deoxyribonuclease I activity: 200-2000 Kunitz units/ml in 10 ml HBSS (#H6648))	specific activity (Collagenase activity : 1-5 units/ml in 10 ml HBSS (#H6648; FALGPA assay)), (Hyaluronidase activity : 80-400 units/ml in 10 ml HBSS (#H6648; Hyaluronidase assay))	specific activity -	specific activity -
form lyophilized powder	form lyophilized powder	form -	form -
storage temp. -10 to -25°C	storage temp. -10 to -25°C	storage temp. 2-8°C	storage temp. 2-8°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 100

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

保管分類

13 - Non Combustible Solids

wgk

WGK 3

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