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FD4

Fluorescein isothiocyanate–dextran

average mol wt 3,000-5,000

Synonym(s):

FITC–Dextran

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100 mg

Available to ship TODAYfromMILWAUKEE

$99.50
250 mg

Available to ship TODAYfromMILWAUKEE

$165.00
1 g

Available to ship TODAYfromMILWAUKEE

$491.00

About This Item

CAS Number:
60842-46-8
UNSPSC Code:
12352201
NACRES:
NA.25
MDL number:
MFCD00131092

$99.50

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biological source

synthetic

Quality Level

100

conjugate

FITC conjugate

form

powder

mol wt

average mol wt 3,000-5,000

extent of labeling

0.001-0.020 mol FITC per mol glucose

technique(s)

HPLC: suitable

color

light yellow to dark orange

solubility

H2O: 25 mg/mL

suitability

suitable for HPLC, suitable for fluorescence indicator, suitable for fluorescent labeling techniques

application(s)

life science and biopharma
metabolomics
microbiology
pharmaceutical

storage temp.

2-8°C

General description

FITC-Dextran, also known as fluorescein isothiocyanate-labeled dextran, is a versatile molecular marker extensively applied in cell and tissue research, encompassing biochemical investigations. This compound is the result of linking the fluorescent FITC dye with dextran, a polysaccharide composed of branching glucose molecules of varied lengths. FITC-Dextrans are instrumental in the examination of cell and tissue permeability, providing vital quantitative data, making them indispensable in both biomedical and biochemical research.

In biochemical investigations focused on the blood-brain barrier (BBB), FITC-Dextran, particularly the variant with an average molecular weight of 4000, holds a central role. It serves as a dependable tool for evaluating the permeability of solutes, ions, and proteins across the BBB, facilitating an improved understanding of the barrier′s integrity and any potential disruptions. This contribution to biochemical exploration is invaluable in shedding light on the brain′s defense mechanisms.

Additionally, FITC-Dextran plays a pivotal role in studies related to cell permeability within the area of biochemical research. This extends to the assessment of BBB permeability and the extent of disruptions within the BBB, aspects that are fundamental to neuroscience and drug delivery research. These biochemical research facets are essential for comprehending disease mechanisms and formulating effective therapeutic strategies.

Furthermore, FITC-Dextran is a cornerstone in cardiovascular, microcirculation, and perfusion studies. It operates as a fluorescent flux tracer compound, allowing for the measurement of critical processes like blood flow, membrane integrity, vascular drainage, and renal elimination. In conclusion, FITC-Dextran proves to be an indispensable asset in the fields of biology, biochemistry, and medicine, offering unparalleled insights into the intricacies of cellular and tissue permeability, the integrity of the blood-brain barrier, and the understanding of diverse physiological and pathological processes.

Application

Dextran labeled with fluorescein isothiocyanate has been used:
  • as a tracer molecule in membrane permeability study[1]
FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. FITC-dextran has been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran.
FITC-dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. Small FITC-dextrans are also used to measure processes such as endocytosis and cell junction permeability. 5kDa FITC-dextran may be used to calibrate microscale diffusion measurements. 5kDa FITC-dextran may be used to measure gut permeability in gut barrier dysfunction research.
Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.

Biochem/physiol Actions

FITC-Dextran serves as a versatile fluorescent tracer extensively used in biological and physiological research. Its primary actions encompass the assessment of permeability in biological barriers, such as the blood-brain barrier, cell membranes, and vascular walls. It is also utilized to investigate cellular uptake mechanisms, endocytosis, and cell junction permeability, focussing on essential cellular processes. FITC-Dextran aids in measuring blood flow, vascular drainage, and renal elimination, providing insights into microcirculation and capillary dynamics. Further, this marker is also instrumental in understanding tissue and organ characteristics and can serve as a valuable tool for tracing molecules, contributing to the exploration of a wide array of biochemical phenomena.

Features and Benefits

  • Suitable for Biochemical and Biomedical research
  • Versatile and adaptable for wide variety of laboratory and research applications

Preparation Note

Sigma typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.

Other Notes

For additional information on our range of Biochemicals, please complete this form.
To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.

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FD20FD40FD150S
Fluorescein isothiocyanate–dextran average mol wt 3,000-5,000

Sigma-Aldrich

FD4

Fluorescein isothiocyanate–dextran

Fluorescein isothiocyanate–dextran average mol wt 20,000

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FD20

Fluorescein isothiocyanate–dextran

Fluorescein isothiocyanate–dextran average mol wt 40,000

Sigma-Aldrich

FD40

Fluorescein isothiocyanate–dextran

Fluorescein isothiocyanate–dextran average mol wt 150,000

Sigma-Aldrich

FD150S

Fluorescein isothiocyanate–dextran

technique(s)

HPLC: suitable

technique(s)

-

technique(s)

-

technique(s)

-

biological source

synthetic

biological source

bacterial (Leuconostoc mesenteroides)

biological source

bacterial (Leuconostoc mesenteroides)

biological source

synthetic

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

200

solubility

H2O: 25 mg/mL

solubility

water: 25 mg/mL

solubility

H2O: 25 mg/mL

solubility

H2O: 25 mg/mL, clear to slightly hazy, yellow-orange

suitability

suitable for HPLC, suitable for fluorescent labeling techniques, suitable for fluorescence indicator

suitability

-

suitability

-

suitability

-

form

powder

form

powder

form

powder

form

powder

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pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCP6809
BCCP6810
BCCN8279
BCCN1673
BCCM7525

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Articles

FITC-labelled polysaccharides

Explore FITC-labelled polysaccharides and their properties, including impact of molecular weight on molecule dimensions.

Related Content

Fluorescein Isothiocyanate-Dextran

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.

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Global Trade Item Number

SKUGTIN
FD4-250MG04061833622452
FD4-1G04061826682609
FD4-100MG04061833622445

Questions

1–4 of 4 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. Hello, I have FITC dextran (FD4 - 100 mg) with a stock concentration of 25 mg/ml. What is the final concentration for a permeability assay (endothelial cells and podocytes in co-culture)?

    1 answer

    1. There isn't one specific final concentration to be used for such an application. It is advised to refer to scientific literature for more details on experimental setup. Below is a citation of an article that could be of potential interest.

      't Hart DC, Yildiz D, Palacio-Castañeda V, Li L, Gumuscu B, Brock R, Verdurmen WPR, van der Vlag J, Nijenhuis T. Co-Culture of Glomerular Endothelial Cells and Podocytes in a Custom-Designed Glomerulus-on-a-Chip Model Improves the Filtration Barrier Integrity and Affects the Glomerular Cell Phenotype. Biosensors (Basel). 2023 Mar 3;13(3):339. doi: 10.3390/bios13030339. PMID: 36979551; PMCID: PMC10046631.

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  4. What is the difference between FD4 and 46944?

    1 answer

    1. Products FD4 and 46944 are essentially the same product, but they are offered for sale by different divisions. The primary difference between the two lies in the amount of FITC conjugated to the dextran (glucose). For FD4, the amount of FITC ranges from 0.001 to 0.020, while for 46944, the range is 0.002 to 0.020. It is important to note that individual lots must fall within these specified minimum and maximum values.

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