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Anti-Myosin Va (LF-18) antibody produced in rabbit

Name: Anti-Myosin Va (LF-18) antibody produced in rabbit
Brand: SIGMA
Price: 628 USD
Availability: InStock

~0.4 mg/mL, affinity isolated antibody, buffered aqueous solution

Enhanced Validation

Synonym(s):

Anti-GS1, Anti-MYH12, Anti-MYO5, Anti-MYR12

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Size/SKU	Availability	Price
0.2 mL	Check Cart for Availability	$628.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD03455613

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ARR, IHC (p), WB

Citations:

$628.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 190 kDa

species reactivity

chicken, rat

enhanced validation

independent
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concentration

~0.4 mg/mL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using rat and chicken cerebellum sections, microarray: suitable, western blot: 1:1,000 using a rat brain extract

UniProt accession no.

Q02440

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MYO5A(4644)
mouse ... Myo5a(17918)
rat ... Myo5a(25017)

Description

General description

Anti-myosin Va (LF-18) recognizes myosin Va (190kDa). Myosin 5a is encoded by the gene mapped to human chromosome 15q21.2. The encoded protein is a member of the unconventional myosin family. It is a two-headed motor containing two identical heavy chains and multiple light chains. Myosin Va is widely expressed, but at high levels in the brain, testes and melanocytes.

Immunogen

synthetic peptide located near the C-terminus of chicken myosin Va (amino acids 1782-1799 with N-terminally added lysine) conjugated to KLH. This sequence is identical in human, mouse and rat.

Application

Anti-Myosin Va (LF-18) antibody produced in rabbit has been used in:

Western blot.
In situ proximity ligation assay (PLA).
Glutathione S-transferase (GST)-spinophilin co-sedimentation assay.

Biochem/physiol Actions

Myosin 5a is an actin-based molecular motor responsible for transport of secretory granules in various cell systems, including insulin granule. Additionally, it also mediates the transport of cargo such as cell-surface receptors, pigment and organelles like endoplasmic reticulum to the plasma membrane. The encoded protein facilitates pigmentation and synaptic activity in the central nervous system. Genetic variations in the gene are associated with the development of immunological and neurological diseases, including Griscelli syndrome type 1 and Elejalde syndrome.

Physical form

Solution in 0.01 M phosphate buffered saline containing 1% BSA and 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8°C for up to one month. For prolonged storage, freeze in working aliquots at -20°C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Other Notes

In order to obtain best results in different techniques and preparations we recommend determining optimal working dilutions by titration test.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	M5062	M0691	M4401
Sigma-Aldrich M4812 Anti-Myosin Va (LF-18) antibody produced in rabbit	Sigma-Aldrich M5062 Anti-Myosin Va (LE-16) antibody produced in rabbit	Sigma-Aldrich M0691 Anti-Myosin VI antibody, Mouse monoclonal	Sigma-Aldrich M4401 Monoclonal Anti-Myosin (Light Chains 20 kDa) antibody produced in mouse
description ~0.4 mg/mL, affinity isolated antibody, buffered aqueous solution	description ~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution	description ~1 mg/mL, clone MUD-19, purified from hybridoma cell culture	description clone MY-21, ascites fluid
biological source rabbit	biological source rabbit	biological source mouse	biological source mouse
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form purified from hybridoma cell culture	antibody form ascites fluid
technique(s) western blot: 1:1,000 using a rat brain extract, microarray: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using rat and chicken cerebellum sections	technique(s) western blot: 1:500 using a rat brain extract, microarray: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using rat and chicken cerebellum sections	technique(s) immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 2 μg/mL using A431 total cell extract, immunohistochemistry: suitable	technique(s) western blot: suitable using denatured and reduced myosin of muscle, indirect immunofluorescence: 1:200 using human or chicken fibroblasts
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

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