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M5909

IgM Isotype Control from murine myeloma

clone MOPC 104E, 200 μg/mL, buffered aqueous solution, purified immunoglobulin

Synonym(s):

Mouse IgM Isotype Control from myeloma

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0.1 mg

Available to ship TODAYfromMILWAUKEE

$169.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00164813
Conjugate:
unconjugated
Clone:
MOPC 104E, monoclonal
Application:
FACS
Citations:
32

$169.00

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biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

clone

MOPC 104E, monoclonal

form

buffered aqueous solution

concentration

200 μg/mL

technique(s)

flow cytometry: suitable

isotype

IgMλ

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

IgM is a glycoprotein antibody that regulates humoral immune responses. Mouse IgM is involved in modulating B cell memory [1]. This antibody isotype has also been implicated in the development of autoimmune responses associated with the pathogenesis of type 1 diabetes in mice [2]. IgM isotype control antibody is specific for anti-mouse whole serum and anti-mouse IgM. The product does not react with antisera to mouse IgA, IgG1, IgG2a, IgG2b, and IgG3.
Isotype controls are non-reactive immunoglobulins of the same isotype as the primary antibody being used in an application. It is recommended that a non-reactive immunoglobulin of the same isotype and concentration be included as a negative control for each monoclonal antibody reagent used in flow cytometry or other immunoassays.
The purified IgM Isotype Control clone MOPC 104E preparation is non-reactive with antisera to mouse IgA, IgG1, IgG2a, IgG2b, and IgG3. When evaluated in flow cytometry, the IgM Isotype Control did not stain human peripheral blood lymphocytes (PBLs). A FITC Goat anti-Mouse IgM (μ-chain specific), Affinity Isolated Antibody (Product No. F 9259) along with 1 μg of the product was incubated with human PBLs and then evaluated by flow cytometry.

Application

IgM Isotype Control from murine myeloma has been used:
  • in chromatin immunoprecipitation (ChIP) assay
  • LABScreen assays
  • immunofluorescent staining

IgM Isotype Control clone MOPC 104E is recommended as a negative control in flow cytometry. Working Concentration: equal concentrations of isotype control and primary antibody are recommended for use in flow cytometry. Equal concentrations of isotype control and primary antibody are recommended for use in flow cytometry.
IgM isotype control antibodies are suitable for use in immunohistochemistry [3][4].
Mouse myeloma proteins tested for use as controls in flow cytometry.

Biochem/physiol Actions

The specificity of staining by monoclonal antibodies to mouse CD antigens should be verified by establishing the non-specific reagent binding to the target cell population. It is recommended that a concentration and isotype matched mouse myeloma immunoglobulin be included as a negative control for each monoclonal antibody reagent used in the immunoassay procedure.
Sigma Mouse Isotype Controls are supplied to match the concentration of Sigma purified and/or conjugated monoclonal antibodies to CD antigens. DUAL-TAG controls contain 100 μg of each conjugate.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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IgM Isotype Control from murine myeloma clone MOPC 104E, 200 μg/mL, buffered aqueous solution, purified immunoglobulin

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Sigma-Aldrich

F6522

IgG2a−FITC Isotype Control from murine myeloma

description

clone MOPC 104E, 200 μg/mL, buffered aqueous solution, purified immunoglobulin

description

clone MOPC 21, purified immunoglobulin, buffered aqueous solution

description

clone MOPC 21, purified immunoglobulin, buffered aqueous solution

description

clone UPC-10, purified immunoglobulin, buffered aqueous solution

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

MOPC 104E, monoclonal

clone

MOPC 21, monoclonal

clone

MOPC 21, monoclonal

clone

UPC-10, monoclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

FITC conjugate

conjugate

FITC conjugate

isotype

IgMλ

isotype

-

isotype

-

isotype

-

technique(s)

flow cytometry: suitable

technique(s)

flow cytometry: suitable

technique(s)

flow cytometry: suitable

technique(s)

flow cytometry: suitable

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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