Skip to Content
MilliporeSigma

Key Documents

View All Documentation

Skip To

S2890

Streptavidin−Alkaline Phosphatase from Streptomyces avidinii

lyophilized powder, ≥700 DEA units/mg protein

Promotion Available

Synonym(s):

Streptavidin-AP, Streptavidin−AP from Streptomyces avidinii

Sign In to View Organizational & Contract Pricing.

Spend $700 — Save 5%; Spend $1,000 — Save 10%; Spend $1,500 — Save 22%*

Sign in to check eligibility. Shop Products

Select a Size

Change View
Size/SKUAvailabilityPrice
250 μg
Please contact Customer Service for Availability
$192.00
1 mg
Please contact Customer Service for Availability
$543.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00132384
Specific activity:
≥700 DEA units/mg protein

$192.00

Save Up To 22%

Please contact Customer Service for Availability
​
Request a Custom Order
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

conjugate

alkaline phosphatase conjugate

Quality Segment

form

lyophilized powder

specific activity

≥700 DEA units/mg protein

composition

Protein, ≥40% biuret

storage temp.

−20°C

General description

Streptavidin, a 58 kDa protein, is a homotetramer purified from Streptomyces avidinii. It has a high affinity for biotin. Streptavidin has been conjugated with either peroxidase or alkaline phosphatase (ALP). These enzymes are used as protein conjugates because of their comparative stability and a wide range of suitable substrates for different applications. ALP conjugates to streptavidin in immunoassays.

Application

The optimal working dilution should be determined empirically using a range of dilutions from a 1 mg/ml stock in buffer.
Streptavidin-alkaline phosphatase from Streptomyces avidinii has been used in enzyme-linked immune absorbent spot (ELISpot) to detect interferon (IFN-γ) producing cells.

Physical form

Lyophilized powder containing trehalose with EPPS, phosphate and traces of magnesium chloride and zinc chloride.

Other Notes

One alkaline phosphatase unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
S5512189730S0677
Streptavidin−Alkaline Phosphatase from Streptomyces avidinii lyophilized powder, ≥700 DEA units/mg protein

Sigma-Aldrich

S2890

Streptavidin−Alkaline Phosphatase from Streptomyces avidinii

Streptavidin−Peroxidase from Streptomyces avidinii lyophilized powder

Sigma-Aldrich

S5512

Streptavidin−Peroxidase from Streptomyces avidinii

Streptavidin

Sigma-Aldrich

189730

Streptavidin

Streptavidin from Streptomyces avidinii recombinant, expressed in E. coli, lyophilized powder

Sigma-Aldrich

S0677

Streptavidin from Streptomyces avidinii

description

lyophilized powder, ≥700 DEA units/mg protein

description

lyophilized powder

description

-

description

recombinant, expressed in E. coli, lyophilized powder

specific activity

≥700 DEA units/mg protein

specific activity

-

specific activity

≥10 units/mg protein

specific activity

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized solid

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200

composition

Protein, ≥40% biuret

composition

Protein, ≥70% E1%/280

composition

-

composition

-

conjugate

alkaline phosphatase conjugate

conjugate

peroxidase conjugate

conjugate

-

conjugate

-

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    101M6256V
    033K9157
    067H8250
    052K9161
    SLBD9281V

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Search for a COA

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Questions

    Reviews

    No rating value

    Active Filters