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Human Vitronectin

Name: Human Vitronectin
Brand: SIGMA
Price: 418 USD
Availability: InStock

recombinant, expressed in HEK 293 cells, powder, suitable for cell culture

Synonym(s):

Epiboin, S-Protein, Serum-spreading factor, V75, VTN

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250 μg	Usually ships in 2 weeks.	$418.00

About This Item

CAS Number:

83380-82-9

UNSPSC Code:

12352202

NACRES:

NA.32

Biological source:

human

Form:

lyophilized

Technique(s):

cell culture | mammalian: suitable

Mol wt:

52.2 kDa (calculated)

Assay:

≥95% (HPLC), ≥95% (SDS-PAGE)

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Properties

Product Name

Vitronectin human, recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

biological source

human

Quality Segment

200

recombinant

expressed in HEK 293 cells

assay

≥95% (HPLC), ≥95% (SDS-PAGE)

form

lyophilized

potency

≤0.5 μg/mL EC₅₀

mol wt

52.2 kDa (calculated)

packaging

pkg of 250 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

solubility

water: soluble

NCBI accession no.

NM_000638.4

UniProt accession no.

P04004

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... VTN(7448)

Description

General description

Vitronectin is a secreted glycoprotein which is synthesized in the liver. It is also secreted by respiratory epithelial cells and is present in healthy lungs. It circulates primarily in monomeric form, but can undergo conformational change to a structure that forms disulfide linked multimers.The vitronectin gene consists of approximately 4.5–5 kb base pairs with eight exons and seven introns.
Recombinant human Vitronectin is a protein consisting of 459 amino acids. It exists in two forms: a single chain and a clipped form consisting of two chains held together by disulfide bonds resulting from an endogenous cleavage. Recombinant vitronectin can function as a chemically defined matrix component in human embryonic stem cell renewal media.

Application

Vitronectin human has been used to study the effects of ECM (extracellular matrix) ligands on C6 astroglioma cells. It has also been used as an attachment-promoting protein for coating the cell plates.

Biochem/physiol Actions

The multimeric vitronectin protein can efficiently bind to and incorporate into the extracellular matrix. Within the matrix, Vitronectin can support cell adhesion through binding to various integrins and other proteoglycans. Its interaction with integrin α_vβ₃ enhances hypoxia resistance and tumor cell invasion. It is also associated with cancer progression in various cancers. It is also involved in wound repair and controls complement system as wel. Moreover, vitronectin functions as a marker of profibrotic activity in liver, heart and kidney.

Features and Benefits

Human-derived vitronectin
Produced through recombinant expression in HEK 293 cells
Low endotoxin levels

Physical form

Lyophilized from Phosphate Buffered Saline (PBS), pH 7.4, plus 2.5% trehalose.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Other Notes

DQESCKGRCT EGFNVDKKCQ CDELCSYYQS CCTDYTAECK PQVTRGDVFT MPEDEYTVYD DGEEKNNATV HEQVGGPSLT SDLQAQSKGN PEQTPVLKPE EEAPAPEVGA SKPEGIDSRP ETLHPGRPQP PAEEELCSGK PFDAFTDLKN GSLFAFRGQY CYELDEKAVR PGYPKLIRDV WGIEGPIDAA FTRINCQGKT YLFKGSQYWR FEDGVLDPDY PRNISDGFDG IPDNVDAALA LPAHSYSGRE RVYFFKGKQY WEYQFQHQPS QEECEGSSLS AVFEHFAMMQ RDSWEDIFEL LFWGRTSAGT RQPQFISRDW HGVPGQVDAA MAGRIYISGM APRPSLAKKQ RFRHRNRKGY RSQRGHSRGR NQNSRRPSRA TWLSLFSSEE SNLGANNYDD YRMDWLVPAT CEPIQSVFFF SGDKYYRVNL RTRRVDTVDP PYPRSIAQYW LGCPAPGHL

Under reducing conditions, the single chain migrates at an apparent molecular mass of 75kDa on SDS-PAGE, and the clipped form migrates at molecular masses of 65kDa and 10kDa.

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This Item	CC080	5051	V0132
Sigma-Aldrich SRP3186 Vitronectin human	Sigma-Aldrich CC080 Vitronectin, Human Purified Protein	Sigma-Aldrich 5051 Vitronectin	Sigma-Aldrich V0132 Vitronectin from rat plasma
description recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture	description -	description from human plasma, suitable for cell culture, solution	description lyophilized powder, BioReagent, suitable for cell culture
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
biological source human	biological source human	biological source human plasma	biological source rat plasma
assay ≥95% (SDS-PAGE), ≥95% (HPLC)	assay ≥90% (SDS-PAGE)	assay >95%	assay -
Quality Level 200	Quality Level 100	Quality Level -	Quality Level 200
mol wt 52.2 kDa (calculated)	mol wt -	mol wt 75 kDa	mol wt 75 kDa
form lyophilized	form liquid	form solution	form lyophilized powder

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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)