SRP3186

Sigma-Aldrich

Vitronectin human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

Synonym(s):
Serum-spreading factor, V75, Epiboin, S-Protein, VTN
CAS Number:
NACRES:
NA.32

Quality Level

biological source

human

recombinant

expressed in HEK 293 cells

sterility

sterile

assay

≥95% (HPLC)
≥95% (SDS-PAGE)

form

lyophilized

potency

≤0.5 μg/mL EC50

mol wt

75 kDa

packaging

pkg of 250 μg

application(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... VTN(7448)

General description

Vitronectin is a secreted glycoprotein which is synthesized in the liver. It is also secreted by respiratory epithelial cells and is present in healthy lungs. It circulates primarily in monomeric form, but can undergo conformational change to a structure that forms disulfide linked multimers. Recombinant human Vitronectin is a 459 amino acid single chain monomeric protein, which migrates at an apparent molecular weight of 75kDa by SDS-PAGE under reducing conditions. Recombinant vitronectin can function as a chemically defined matrix component in human embryonic stem cell renewal media.

Application

Vitronectin human has been used to study the effects of ECM (extracellular matrix) ligands on C6 astroglioma cells. It has also been used as an attachment-promoting protein for coating the cell plates.

Biochem/physiol Actions

The multimeric Vitronectin can efficiently bind to and incorporate into the extracellular matrix. Within the matrix, Vitronectin can support cell adhesion through binding to various integrins and other proteoglycans. Its interaction with integrin αvβ3 enhances hypoxia resistance and tumor cell invasion. It is also associated with cancer progression in various cancers. Vitronectin is involved in wound repair and also controls complement system. It works as a marker of profibrotic activity in liver, heart and kidney.

Sequence

DQESCKGRCT EGFNVDKKCQ CDELCSYYQS CCTDYTAECK PQVTRGDVFT MPEDEYTVYD DGEEKNNATV HEQVGGPSLT SDLQAQSKGN PEQTPVLKPE EEAPAPEVGA SKPEGIDSRP ETLHPGRPQP PAEEELCSGK PFDAFTDLKN GSLFAFRGQY CYELDEKAVR PGYPKLIRDV WGIEGPIDAA FTRINCQGKT YLFKGSQYWR FEDGVLDPDY PRNISDGFDG IPDNVDAALA LPAHSYSGRE RVYFFKGKQY WEYQFQHQPS QEECEGSSLS AVFEHFAMMQ RDSWEDIFEL LFWGRTSAGT RQPQFISRDW HGVPGQVDAA MAGRIYISGM APRPSLAKKQ RFRHRNRKGY RSQRGHSRGR NQNSRRPSRA TWLSLFSSEE SNLGANNYDD YRMDWLVPAT CEPIQSVFFF SGDKYYRVNL RTRRVDTVDP PYPRSIAQYW LGCPAPGHL

Physical form

Lyophilized from Phosphate Buffered Saline (PBS), pH 7.4, plus 2.5% trehalose.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Vitronectin expression in the airways of subjects with asthma and chronic obstructive pulmonary disease.
Salazar-Pelaez LM, et al.
PLoS ONE, 10, e0119717-e0119717 (2015)
Extracellular matrices and cell adhesion.
E Ruoslahti et al.
Arteriosclerosis (Dallas, Tex.), 5(6), 581-594 (1985-11-01)
Carine Ysebaert et al.
Infection and immunity, 87(8) (2019-05-22)
PE-PilA is a fusion protein composed of immunologically relevant parts of protein E (PE) and the majority subunit of the type IV pilus (PilA), two major antigens of nontypeable Haemophilus influenzae (NTHi). Here we report on the preclinical evaluation of...
Distribution of vitronectin mRNA during murine development.
Seiffert D, et al.
Developmental Dynamics, 203, 71-79 (1995)
Degradation of distinct forms of multimeric vitronectin by human fibroblasts.
Wilkins-Port CE and McKeown-Longo PJ
Biochimica et Biophysica Acta, 1404, 353-366 (1998)
Protocols
Vitronectin is used to coat culture vessels for cells attachment.
Read More
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