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Monoclonal Anti-Talin antibody produced in mouse

Name: Monoclonal Anti-Talin antibody produced in mouse
Brand: SIGMA
Price: 552 USD
Availability: InStock

clone 8d4, ascites fluid

Synonym(s):

Monoclonal Anti-Talin

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Size/SKU	Availability	Price
100 μL	Available to ship TODAYfromMILWAUKEE	$552.00
0.2 mL	Available to ship TODAYfromMILWAUKEE	$763.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00164744

Conjugate:

unconjugated

Clone:

8d4, monoclonal

Application:

IF, IP, WB

Citations:

191

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

8d4, monoclonal

mol wt

antigen 225-235 kDa

contains

15 mM sodium azide

species reactivity

chicken, frog, rat, human, mouse

technique(s)

immunoprecipitation (IP): suitable, indirect immunofluorescence: 1:500 using cultured Rat2 cells, western blot: 1:100 using chicken gizzard extract

isotype

IgG1

UniProt accession no.

Q9Y490

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TLN1(7094), TLN2(83660)
mouse ... Tln1(21894), Tln2(70549)
rat ... Tln1(313494)

Description

General description

Monoclonal Anti-Talin (mouse IgG1 isotype) is derived from the 8d4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Talin is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin.

Talin 1 (TLN1) gene mapped to human chromosome 9p13.3, codes for a 270kDa cytoskeletal protein containing 2541 amino acids. Talin-1 is widely expressed in the kidney, liver, spleen, stomach, lung and vascular smooth muscle. The TLN1 antiparallel dimeric structure is characterized with a globular head containing four-point-one, ezrin, radixin, moesin (FERM) domain and rod domain involved in dimer formation.

Immunogen

purified chicken gizzard talin.

Application

Monoclonal Anti-Talin may be used for the localization of talin using various immunochemical assays such as immunoblot, immunocytochemistry, immunofluorescence, enzyme linked immunosorbent assay (ELISA) and for microinjection and transfection studies. The antibody recognizes native talin and can be used for immunoprecipitation.

Biochem/physiol Actions

Reacts specifically with mammalian and avian talin. In immunoblotting, the antibody recognizes an epitope located on the 225 kDa chicken talin molecule and the 190 kDa fragment obtained by protease cleavage. It also localizes the slightly higher molecular weight talin in human platelets. The antibody labels focal adhesions, membrane ruffles, and ventral streaks in methanol/acetone fixed cultured fibroblasts.

Talin 1 (TLN1) is a cytoskeletal protein, which plays a vital role in cancer cell adhesion, migration and invasion. It is also involved in integrin activation and it facilitates the adhesion of integrin to actin cytoskeleton. TLN1 might act as a potential prognostic biomarker in nasopharyngeal carcinoma (NPC). Overexpression of TLN1 is observed in endometrioid carcinoma, oral squamous cell carcinoma (OSCC), prostate cancer and hepatocellular carcinoma. Serum TLN1 functions as a potential biomarker of hepatocellular carcinoma (HCC). Talin 1 (TLN1) is highly expressed in glioblastoma multiforme (GBM). The Inhibition of TLN1 activity helps in diminishing malignant features of GBM cells. It also plays an important role in regulation of, Bevacizumab (anti-angiogenic agent)-resistance.

Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin. Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, between the extracellular matrix and the cytoskeleton, that occurs at adhesion plaques.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB4200041	SAB4200694	P1093
Sigma-Aldrich T3287 Monoclonal Anti-Talin antibody produced in mouse	Sigma-Aldrich SAB4200041 Anti-Talin antibody,Mouse monoclonal	Sigma-Aldrich SAB4200694 Anti-Talin antibody, Mouse monoclonal	Sigma-Aldrich P1093 Monoclonal Anti-Paxillin antibody produced in mouse
description clone 8d4, ascites fluid	description clone TA205, purified from hybridoma cell culture	description clone 8D4, purified from hybridoma cell culture	description clone PXC-10, ascites fluid
clone 8d4, monoclonal	clone TA205, monoclonal	clone 8D4, monoclonal	clone PXC-10, monoclonal
antibody form ascites fluid	antibody form purified from hybridoma cell culture	antibody form purified immunoglobulin	antibody form ascites fluid
biological source mouse	biological source mouse	biological source mouse	biological source mouse
species reactivity chicken, frog, rat, human, mouse	species reactivity chicken, bovine, human	species reactivity Xenopus, rat, chicken, human, bovine, sheep, hamster	species reactivity bovine, rat, human, chicken, hamster
conjugate unconjugated	conjugate unconjugated	conjugate -	conjugate unconjugated
Gene Information human ... TLN1(7094), TLN2(83660) mouse ... Tln1(21894), Tln2(70549) rat ... Tln1(313494)	Gene Information chicken ... Tln1(395194) human ... TLN1(7094)	Gene Information chicken ... Tln1(395194) human ... TLN1(7094) rat ... Tln1(313494)	Gene Information human ... PXN(5829) rat ... Pxn(360820)

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c