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T8154

Trypan Blue solution

Name: Trypan Blue solution
Brand: SIGMA

0.4%, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Blue cell viability dye

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About This Item

Empirical Formula (Hill Notation):

C₃₄H₂₄N₆Na₄O₁₄S₄

CAS Number:

72-57-1

Molecular Weight:

960.81

UNSPSC Code:

12352207

NACRES:

NA.75

PubChem Substance ID:

57654704

MDL number:

MFCD00003969

Beilstein/REAXYS Number:

4360496

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Properties

sterility

sterile-filtered

Quality Segment

200

form

liquid

storage condition

dry at room temperature

concentration

0.4%

technique(s)

cell culture | mammalian: suitable, tissue processing: suitable

application(s)

cell analysis

shipped in

ambient

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

Description

General description

For the same product in a convenient lyophilized (powder) format, please see T6146

Trypan Blue (TB), an anionic hydrophilic azo dye, crosses only the cell membranes of dead cells, thereby staining dead tissues/cells blue. It is used in quantitative microscopy for a dye exclusion staining technique to differentiate between live and dead mammalian cells.

Trypan blue is a dye used to distinguish between live and dead cells. It is a vital stain that is not absorbed by healthy viable cells, but stains cells with a damaged cell membrane. This way, only dead cells can be counted. The method is sometimes referred to as the dye exclusion method.

Application

Trypan Blue solution has been used:

in cell viability assay to count viable and dead cells
in rescue assay to count viable cells
in trypan blue exclusion counting method to determine proliferation curve
as the saline control and in the preparation of lipopolysaccharide (LPS) solution to confirm the success of LPS infusion
in trypan blue dye exclusion test to determine gastric epithelial cells (GEC) viability
to count GEC cells using a hemocytometer

Preparation Note

Prepared in 0.81% sodium chloride and 0.06% potassium phosphate, dibasic.

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Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H350

Precautionary Statements

P201 - P202 - P280 - P308 + P313 - P405 - P501

Hazard Classifications

Carc. 1B

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Flash Point (°F)

Not applicable

Flash Point (°C)