dry at room temperature
cell analysis: suitable
cell culture | mammalian: suitable
tissue processing: suitable
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NONH for all modes of transport
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
Trypan Blue has a greater affinity for serum proteins than for cellular protein. If the background is too dark, cells should be pelleted and resuspended in protein-free medium or salt solution prior to counting.
Trypan Blue is a vital stain recommended for use in estimating the proportion of viable cells in a population. The reactivity of this dye is based on the fact that the chromophore is negatively charged and does not react with the cell unless the membrane is damaged. Staining facilitates the visualization of cell morphology. Live (viable) cells do not take up the dye and dead (non-viable) cells do.
Trypan blue will stain cells that have disrupted membranes. It cannot differentiate between permeabilized membranes caused by apoptosis or necrosis.
The trypan blue solution is still good to use. It can be warmed to dissolve the precipitate or it can be filtered to remove the precipitate.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
A report of fibers in Product T8154, Trypan blue solution is sufficiently common almost to be a characteristic of the product. These fibers are a cosmetic fault rather than one of functionality. The presence of fibers may be more pronounced following cold storage or shipping. This product should be stored at room temperature. Filtration to remove the fibers will not affect the use of the product.
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