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T8154

Sigma-Aldrich

Trypan Blue solution

0.4%, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
Blue cell viability dye
Empirical Formula (Hill Notation):
C34H24N6Na4O14S4
CAS Number:
Molecular Weight:
960.81
Beilstein/REAXYS Number:
4360496
MDL number:
PubChem Substance ID:
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

form

liquid

storage condition

dry at room temperature

concentration

0.4%

technique(s)

cell culture | mammalian: suitable
tissue processing: suitable

application(s)

cell analysis

shipped in

ambient

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

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This Item
93595T61461.04192
Trypan Blue solution 0.4%, liquid, sterile-filtered, suitable for cell culture

T8154

Trypan Blue solution

Trypan Blue solution 0.4%, for microscopy

93595

Trypan Blue solution

Trypan Blue powder, BioReagent, suitable for cell culture

T6146

Trypan Blue

Trypan Blue

1.04192

Trypan Blue

concentration

0.4%

concentration

0.4%

concentration

-

concentration

-

form

liquid

form

liquid

form

powder

form

solid

application(s)

cell analysis

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

cell analysis

application(s)

diagnostic assay manufacturing
hematology
histology

shipped in

ambient

shipped in

-

shipped in

-

shipped in

-

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200

General description

Trypan Blue (TB), an anionic hydrophilic azo dye, crosses only the cell membranes of dead cells, thereby staining dead tissues/cells blue. It is used in quantitative microscopy for a dye exclusion staining technique to differentiate between live and dead mammalian cells.
Trypan blue is a dye used to distinguish between live and dead cells. It is a vital stain that is not absorbed by healthy viable cells, but stains cells with a damaged cell membrane. This way, only dead cells can be counted. The method is sometimes referred to as the dye exclusion method.

Application

Trypan Blue solution has been used:
  • in cell viability assay to count viable and dead cells
  • in rescue assay to count viable cells
  • in trypan blue exclusion counting method to determine proliferation curve
  • as the saline control and in the preparation of lipopolysaccharide (LPS) solution to confirm the success of LPS infusion
  • in trypan blue dye exclusion test to determine gastric epithelial cells (GEC) viability
  • to count GEC cells using a hemocytometer

Preparation Note

Prepared in 0.81% sodium chloride and 0.06% potassium phosphate, dibasic.

also commonly purchased with this product

recommended

Product No.
Description
Pricing

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Carc. 1B

Storage Class

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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