ZytoLight^® SPEC MYB Dual Color Break Apart Probe

The ZytoLight^® SPEC MYB Dual Color Break Apart Probe (PL100) is a direct-labeled FISH probe for the qualitative detection of translocations of the human MYB gene at 6q23.3 in cytologic or formalin-fixed, paraffinembedded specimens, using fluorescence in situ hybridization (FISH). The probe is intended for use with ZytoLight FISH Implementation Kits.

ZytoLight^® probes enable the precise identification of genetic abnormalities, including translocations, deletions, amplifications, and chromosomal aneuploidies, in formalin-fixed, paraffin-embedded (FFPE) tissue sections or cytology specimens, without the need for additional antibody-based detection.

Components

The ZytoLight SPEC MYB Dual Color Break Apart Probe comprises:

• ZyGreen (excitation 503 nm/emission 528 nm) labeled polynucleotides (~10.0 ng/µl), targeting sequences mapping in 6q23.2-q23.3* (chr6:134,840,690-135,483,752) proximal to the MYB breakpoint region;
• ZyOrange (excitation 547 nm/emission 572 nm) labeled polynucleotides (~4.5 ng/µl), targeting sequences mapping in 6q23.3* (chr6:135,728,667-136,390,142) distal to the MYB breakpoint region;
• Formamide based hybridization buffer

*according to Human Genome Assembly GRCh37/hg19

Principle

The fluorescence in situ hybridization (FISH) method enables the identification and visualization of particular nucleic acid sequences within cell samples. DNA fragments labeled with fluorescent dyes, known as FISH probes, are co-denatured with their complementary target DNA strands in the sample and then allowed to hybridize. Following hybridization, non-specific and unbound probe fragments are removed through stringency washes. The DNA is then counterstained with DAPI, and the hybridized probes are detected using a fluorescence microscope equipped with filters matched to the specific fluorochromes used to label the FISH probes.

This probe is intended for use in cytologic or formalin-fixed, paraffin-embedded specimens, to qualitatively detect translocations of the human MYB gene at 6q23.3, via FISH.

Using suitable filter sets, the probe hybridization signals of the appear green near the MYB breakpoint region and orange away from it.

• Normal scenario: in normal cells during interphase or cells without an MYB gene region translocation, two green/orange fusion signals are observed.
• Aberrant scenario: one translocated MYB gene region is shown by one separate green and orange signal each.

Overlapping signals may appear yellow.

ZytoLight is a registered trademark of ZytoVision GmbH in Germany