159220

Diethylpyrocarbonat

96% (NT)

• Synonym(e): DEP, DEPC, Diethyldicarbonat, Diethyloxydiformat, Ethoxy-Ameisensäure-Anhydrid
• Lineare Formel: O(COOC₂H₅)₂
• CAS-Nummer: 1609-47-8
• Molekulargewicht: 162.14
• UNSPSC Code: 12352100
• NACRES: NA.22
• PubChem Substance ID: 24849799
• EC Number: 216-542-8
• Beilstein/REAXYS Number: 637031
• MDL number: MFCD00009106

Eigenschaften

• Produktname: Diethylpyrocarbonat, 96% (NT)
• InChI key: FFYPMLJYZAEMQB-UHFFFAOYSA-N
• InChI: 1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3
• SMILES string: CCOC(=O)OC(=O)OCC
• assay: 96% (NT)
• form: liquid
• refractive index: n20/D 1.398 (lit.)
• bp: 93-94 °C/18 mmHg (lit.)
• density: 1.101 g/mL at 25 °C (lit.)
• functional group: carbonate
• shipped in: wet ice
• storage temp.: 2-8°C
• Quality Level: 200

Beschreibung

Application

Modifizierungsreagenz für His- und Tyr-Rückstände in Proteinen. Stabile Sonde für Strukturstörungen in dsDNA durch Reaktion mit voll oder teilweise entstapelten Basen.

Diethyl pyrocarbonate was used as:

• nuclease inhibitor for the extraction of undegraded nucleic acids from etiolated and green plant tissues
• an antimicrobial additive
• ribonuclease inhibitor
• histidine residue modifier
• reagent for conversion of imines to carbamates

Biochem/physiol Actions

Inaktiviert RNase in Lösung bei ca. 0,1 % (v/v) und bietet RNA damit Schutz vor Zersetzung.

General description

Diethyl pyrocarbonate reacts selectively with the single-stranded loops of cruciform structures. The irreversible inactivation of ribonuclease with diethylpyrocarbonate has been studied.

Sicherheitshinweise

• pictograms: GHS07
• signalword: Warning
• hcodes: H302
• pcodes: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: 156.2 °F - closed cup
• flash_point_c: 69 °C - closed cup
• ppe: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter