During Quality Control, no testing to performed involving confocal microscopy. Conjugate 5-aminofluorescein (AFL)-human serum albumin has been used to stain brain cells, although it does not appear confocal microscopy was performed. Below is the reference: https://www.sigmaaldrich.com/tech-docs/paper/250406
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201626
Fluoreszeinamin, Isomer I
Synonym(e):
5-Aminofluorescein
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 g | Warenkorb auf Verfügbarkeit prüfen | € 197,00 |
5 g | Warenkorb auf Verfügbarkeit prüfen | € 676,00 |
Über diesen Artikel
Empirische Formel (Hill-System):
C20H13NO5
CAS-Nummer:
Molekulargewicht:
347.32
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
222-043-6
Beilstein/REAXYS Number:
48395
MDL number:
Technischer Dienst
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Unterstützung erhaltenform
powder
Quality Segment
mp
223 °C (dec.) (lit.)
solubility
methanol: 5 mg/mL
λmax
490-501 nm
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
Nc1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35
InChI
1S/C20H13NO5/c21-10-1-4-14-13(7-10)19(24)26-20(14)15-5-2-11(22)8-17(15)25-18-9-12(23)3-6-16(18)20/h1-9,22-23H,21H2
InChI key
GZAJOEGTZDUSKS-UHFFFAOYSA-N
General description
Fluoresceinamine, isomer I belongs to the class of derivatized fluoresceins.
Application
Fluoresceinamine, isomer I is suitable for use in a specific and sensitive spectrophotometric method for determining nitrite. It has been used to fluorescently tag nanoparticles through a competitive carboxyl-amine coupling reaction to visualize nanoparticle internalization.
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Dieser Artikel | |||
|---|---|---|---|
| description - | description BioReagent, suitable for fluorescence, ~95% (HPLC) | description suitable for protein labeling, ≥90% (HPLC), powder | description ≥97.5% (HPLC) |
| form powder | form powder | form powder | form powder |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| solubility methanol: 5 mg/mL | solubility DMSO: soluble, acetone: soluble, methanol: soluble | solubility acetone: 1 mg/mL | solubility acetone: soluble 10 mg/mL |
| storage temp. room temp | storage temp. - | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
| mp 223 °C (dec.) (lit.) | mp 285 °C (dec.) (lit.) | mp >360 °C (lit.) | mp >360 °C (lit.) |
Signalwort
Warning
Gefahrencodes
Sicherheitshinweise
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Zielorgane
Respiratory system
Lagerklasse
11 - Combustible Solids
Was ist los?
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
PSA (Persönliche Schutzausrüstung)
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Can this be used to stain cells and visualize them under the confocal microscope?
1 answer-
Helpful?
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Dear manager, I want to ask you 2 questions about this product. 1. Is it a flurescence dye? Can you show me its adsorption or emission spectrum? 2. Is the amine group reactive?
1 answer-
Yes, Fluoresceinamine, isomer I is classified as belonging to a group of derivatized fluorosceins. The product is fluorescent. Although no quality control testing is performed to check for excitation and emission, the dye is expected to excite around 490 nm and emit around 520 nm.
As the H2N- group is not acetylated or protonated, the amine group can be considered reactive. There is literature available stating Fluoresceinamine, isomer I can be covalently bound to human serum albumin.
Helpful?
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What is the solubility of Fluoresceinamine, isomer I, CAS 3326-34-9?
1 answer-
Soluble in methanol at a concentration of 5 mg/mL. Soluble in DMSO at a concentration of ≥ 32 mg/mL.
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