14-902-M

AMPK (α2, β1, γ1) Protein, active, 10 µg

Active, recombinant, human AMPK (α2, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα2, full length untagged AMPKβ1 & full length untagged AMPKγ1. For use in Kinase Assays.

• UNSPSC Code: 12352202
• NACRES: NA.32
• eCl@ss: 32160405
• Biological source: human
• Recombinant: expressed in E. coli
• Mol wt: M_w 30 kDa (AMPKβ1), M_w 38 kDa (AMPKγ1), M_w 63 kDa (AMPKα2)

Eigenschaften

• biological source: human
• Quality Segment: 100
• recombinant: expressed in E. coli
• product line: Upstate^®
• specific activity: (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)
• mol wt: M_w 30 kDa (AMPKβ1), M_w 38 kDa (AMPKγ1), M_w 63 kDa (AMPKα2)
• purified by: (Ni²⁺/NTA-agarose)
• species reactivity: human
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• accession no.: (Function: Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5′-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. ), NM_006252
• NCBI accession no.: NM_002733, NM_006252, NM_006253
• UniProt accession no.: P54619, P54646, P54646, Q9Y478, P54619, Q9Y478
• relevant disease(s): diabetes; obesity; metabolic disorders
• Gene Information: human ... PRKAA2(5563), PRKAB1(5563)

Beschreibung

General description

AMPK (α2, β1, γ1)

Recombinant, human AMPK (α2, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα2, full length untagged AMPKβ1 and full length untagged AMPKγ1.

Application

Research Category
Metabolism

Apoptosis & Cancer

Research Sub Category
Diabetes

Obesity

Metabolic Disorders

Biochem/physiol Actions

Protein Target: AMPK (α2, β1, γ1)

Target Sub-Family: CAMK

Packaging

Also available in 10 μg size and in bulk

Also available in 250µg size as catalog # 14-902M - Call for pricing and availability.

Physical form

Cobalt-agarose

Preparation Note

6 months at -70°C

Analysis Note

Routinely evaluated by phosphorylation of substrate peptide

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• Gefahrencodes: H317
• Sicherheitshinweise: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Lagerklasse: 10 - Combustible liquids
• Was ist los?: WGK 1
• Flammpunkt (°C): Not applicable