The recommended dilution for 20-116 Sigma-Aldrich Kinase Inhibitor Cocktail is 1:5, preferably in the cell extraction buffer, so it is already present during lysis. Here are a couple of articles where the inhibitor cocktail has been used:
- Carcinogenesis. 2007 Jul;28(7):1408-17. Epub 2007 Mar 19.
- Carcinogenesis. 2008 Mar;29(3):473-9. Epub 2007 Oct 4.
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Produktname
Kinase-Inhibitor-Cocktail, The Inhibitor Cocktail controls the biological activity of kinases. This small molecule/inhibitor is primarily used for Biochemicals applications.
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form
liquid
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
input
general use sample(s)
solubility
water: soluble
shipped in
wet ice
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| description The Inhibitor Cocktail controls the biological activity of kinases. This small molecule/inhibitor is primarily used for Biochemicals applications. | description DMSO solution | description - | description aqueous solution (dark coloration may develop upon storage, which does not affect the activity) |
| form liquid | form DMSO solution | form liquid | form aqueous solution (dark coloration may develop upon storage, which does not affect the activity) |
| manufacturer/tradename Upstate® | manufacturer/tradename - | manufacturer/tradename Upstate® | manufacturer/tradename - |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 300 |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| solubility water: soluble | solubility water: soluble | solubility - | solubility water: soluble |
| input general use sample(s) | input - | input - | input general use sample(s) |
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12 - Non Combustible Liquids
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Not applicable
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What dilution do you recommend for cell culture for this product?
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